| 区分土壤中硝化与反硝化对N2O产生贡献的方法 |
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| 引用本文： | 黄树辉,吕军.区分土壤中硝化与反硝化对N2O产生贡献的方法[J].农业工程学报,2005,21(13):48-51. |
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| 作者姓名： | 黄树辉 吕军 |
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| 作者单位： | 浙江大学环境与资源学院，杭州 310029;浙江大学环境与资源学院，杭州 310029 |
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| 基金项目： | 973项目(2002CB410807)；国家自然科学基金(40171047) |
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| 摘 要： | 土壤是产生N2O的最主要来源之一。硝化和反硝化反应是产生N2O的主要机理，由于硝化和反硝化微生物同时存在于土壤中，因而硝化和反硝化作用能同时产生N2O。N2O的来源可通过使用选择性抑制剂，杀菌剂以及加入的标记底物确定。通过对生成N2O反应的每一步分析，主要从抑制反应发生的催化酶和细菌着手，总结了测量区分硝化、反硝化和DNRA反应对N2O产生的贡献方法。并对15N标记底物法，乙炔抑制法和环境因子抑制法作了详细介绍。
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| 关 键 词： | 土壤 氧化亚氮 硝化与反硝化 N2O |
| 收稿时间： | 2003/10/13 0:00:00 |
| 修稿时间： | 4/5/2004 12:00:00 AM |
Methods for the contributions of nitrification and denitrification to the production of nitrous oxide from soil |
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| Abstract: | Most of nitrous oxide produced from soil. The flux of N2O from soil can be resulted from nitrification, denitrification or dissimilatory NO-3 reduction to NH+4(DNRA), and nitrification and denitrification are the primary mechanism about N2O forming. Since aerobic and anaerobic microsites can develop within the same soil, nitrification and denitrification could be occurring at the same time. Their contributions to nitrous oxide emission could differentiate from inhibiting catalysis enzymes and bacteria from each reaction process. Some methods such as 15N-labelling the substrate, acetylene inhibition and environmental factors inhibition are introduced in detail. |
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| Keywords: | soil nitrous oxide nitrification and dentrification N2O |
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