| 梨自交不亲和强度不同品种花柱S糖蛋白含量的差异 |
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| 引用本文： | 张绍铃,杨记磙,李秀根,平塚伸.梨自交不亲和强度不同品种花柱S糖蛋白含量的差异[J].园艺学报,2002,29(2):168-167. |
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| 作者姓名： | 张绍铃 杨记磙 李秀根 平塚伸 |
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| 作者单位： | ( 南京农业大学园艺学院, 南京210095; 中国农业科学院郑州果树研究所, 郑州450009; 三重大学生物资源学部,日本, 津市514􀀁8507 ) |
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| 基金项目： | 国家自然科学基金资助项目 ( 30 170 6 5 1)，江苏省自然科学基金资助项目 (BK2 0 0 10 6 2 )，高校博士学科点专项科研基金资助项目 ( 2 0 0 10 30 70 12 ) |
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| 摘 要： | 提取梨( Pyrus) 自交不亲和强度不同品种花柱可溶性蛋白质, 应用等电聚焦电泳法在凝胶板上分离出S 糖蛋白, 用图像解析法进行定量。结果表明, 花柱总S 糖蛋白含量因品种而异, 自交不亲和强、弱及自交亲和的品种每1 􀀁g 花柱可溶性蛋白中含总S 糖蛋白分别为􀀂1. 3 ng 、0. 7 ~ 0. 9 ng、 0. 7 ng 。不同的S 基因所表达的S 糖蛋白量差异显著, 即使是同一S 基因所对应的S 糖蛋白量也因品种而异。
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| 关 键 词： | 梨 自交不亲和性 S 基因 S 糖蛋白 |
| 文章编号： | 0513-353X(2002)02-0165-03 |
| 修稿时间： | 2001年7月23日 |
Differences of S-glycoprotein Content in the Styles among Pear Cultivars Differing in Self-incompatible Strength |
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| Joseph Ngwela Wolukau.Differences of S-glycoprotein Content in the Styles among Pear Cultivars Differing in Self-incompatible Strength[J].Acta Horticulturae Sinica,2002,29(2):168-167. |
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| Authors: | Joseph Ngwela Wolukau |
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| Institution: | ( College of Horticulture, Nanjing Agriculture University , Nanj ing 210095, China; Zhengz hou Fruit Research I nstitute , CAAS, Zhengzhou 450009, China; Faculty of Bioresources , Mie University , Tsu 5148507, Japan) |
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| Abstract: | Stylar soluble proteins were extracted from the whole style of pear cultivars differing in self&;#1048577;incom patibility ( SI) strength and were separated by isoelectricfocusing polyacrylamide gel electrphoresis ( IEF&;#1048577;PAGE) and each S-glycoprotein content was quantif ied by an image analyzer. Total S-glycoprotein ( sum of two allelic&;#1048577;linked S glycoproteins) content differed among cultivars. The cult ivars which were strong SI, weak SI and sel&;#1048577;compatibility contained &;#1048578;1. 3 ng, 0. 7 - 0. 9 ng, and 0. 7 ng of S&;#1048577;glycoprotein per &;#1048577;g of stylar soluble protein, respectively. S-allele linked glycoprotein content differed greatly in various S-genotypes. Furthermore, the S-glycoprotein content was even different in cultivars with the same S genotype. |
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| Keywords: | Pear ( Pyrus ) Self-incompatibility S -allele S-glycoproteins |
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