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重庆产泽漆中总黄酮与芦丁的含量分析
引用本文:张丹,郑秀云,颜学伟,曹纬国,王刚.重庆产泽漆中总黄酮与芦丁的含量分析[J].安徽农业科学,2011,39(20):12108-12110.
作者姓名:张丹  郑秀云  颜学伟  曹纬国  王刚
作者单位:1. 重庆医科大学中医药学院,重庆,401331
2. 四川省邛崃市医疗中心医院设备科,四川,邛崃,611530
3. 重庆医科大学中医药学院,重庆,401331;重庆医科大学中医药研究室,重庆,400016;重庆医科大学中医药实验教学中心,重庆,401331
基金项目:重庆市卫生局科研项目,重庆市渝中区科技创新项目
摘    要:目的]建立泽漆中总黄酮和芦丁含量测定方法,为进一步开发利用泽漆资源并对其进行质量控制提供科学依据。方法]分别采用三氯化铝比色法和HPLC法测定泽漆中总黄酮和泽漆中芦丁的含量。结果]重庆产区泽漆总黄酮的含量为5.485~5.742 mg/g,芦丁的含量为0.769~0.811 mg/g。结论]该方法简便、快速、准确、稳定,适用于泽漆中总黄酮和芦丁的含量测定。

关 键 词:泽漆(Ephorbia  helioscopia  L.)  总黄酮  芦丁  HPLC  比色法

Determination of Total Flavonoids and Rutin in Ephorbia helioscopia L.from Chongqing
Institution:ZHANG Dan et al(College of Traditional Chinese Medicine,Chongqing University of Medical Sciences,Chongqing 401331)
Abstract:Objective] To establish methods for determining contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing,so as to provide a scientific basis for further development and utilization of its resources and quality control.Methods] Contents of total flavonoids and rutin were respectively determined by aluminium trichloride colorimetric method and HPLC.Result] Contents of total flavonoids and rutin in Ephorbia helioscopia L.from Chongqing were 5.485-5.742 and 0.769-0.811 mg/g,respectively.Conclusion] The method was simple,rapid,accurate and stable that was suitable for quality analysis of total flavonoids and rutin in Ephorbia helioscopia L..
Keywords:Ephorbia helioscopia L    Total flavonoids  Rutin  HPLC  Colorimetry
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