| HP20树脂分离纯化赤芍抑菌活性成分的研究 |
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| 引用本文： | 苏显中,于敏,李明,郑全喜,刘守坤.HP20树脂分离纯化赤芍抑菌活性成分的研究[J].安徽农业科学,2011,39(20):12067-12069. |
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| 作者姓名： | 苏显中 于敏 李明 郑全喜 刘守坤 |
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| 作者单位： | 中国矿业大学化工学院生物工程系,江苏,徐州,221116 |
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| 基金项目： | 教育部留学回国人员科研启动基金，徐州市科技计划项目 |
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| 摘 要： | 目的]从赤芍中提取并确定主要抑菌活性成分。方法]采用乙醇溶液超声波提取、萃取、HP20树脂柱层析等工序处理赤芍,并将提取物对肺炎克雷伯菌、鲍曼不动杆菌和黏质沙雷氏菌采用液体2倍稀释法进行体外抑菌试验;用傅里叶变换红外光谱仪分析提取物的FTIR光谱。结果]100%乙醇洗脱液对3种供试细菌的抑制效果最好,最小抑制浓度（MIC）分别为32.00、32.00和125.00 g/L;提取物与芍药苷标准品的红外光谱相似。结论]芍药苷是赤芍的主要活性成分,具有明显的抑菌作用。
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| 关 键 词： | 赤芍 活性成分 傅里叶变换红外光谱 抑菌活性 |
Study on the Extraction and Purification of the Antimicrobial Active Constituents from RADIX PAEONIAE RUBRA by Using HP20 Resin |
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| Institution: | SU Xian-zhong et al(Department of Biological Engineering,School of Chemical Engineering,China University of Mining and Technology,Xuzhou,Jiangsu 221116) |
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| Abstract: | Objective] To extract and determine the major antimicrobial active constituents from RADIX PAEONIAE RUBRA.Method] The active constituents of RADIX PAEONIAE RUBRA was firstly prepared with 90% ethanol,refined with other chemicals and then purified with HP20 resin,and the in vitro antimicrobial effect of the two-fold dilution of extracts was experimented against Klebsiella pneumoniae ATCC 49790,Acinetobacter baumannii 36 and Serratia marcescens ATCC 14041.The FTIR spectra of the extracts were analyzed.Result] The antimicrobial activity of the 100% elution extract of RADIX PAEONIAE RUBRA was the highest and the MICs were 32.00,32.00 and 125.00 g/L respectively;the FTIR spectra of the extracts was very similar to that of peoniflorin.Conclusion] Peoniflorin was the main active constituents of RADIX PAEONIAE RUBRA,it had significant antimicrobial effect. |
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| Keywords: | RADIX PAEONIAE RUBRA Extract FTIR spectra Antimicrobial activity |
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