| SARS病毒S蛋白S1和S2片段的合成及在大肠杆菌中的表达 |
| |
| 引用本文： | 郑尚永,潘卫庆.SARS病毒S蛋白S1和S2片段的合成及在大肠杆菌中的表达[J].安徽农业科学,2011,39(20):12208-12211. |
| |
| 作者姓名： | 郑尚永 潘卫庆 |
| |
| 作者单位： | 中国人民解放军第二军医大学病原生物教研室,上海,200433 |
| |
| 摘 要： | 目的]通过拼接完成对SARS S蛋白的上下游序列S1和S2的合成,并在大肠杆菌中表达,获得具活性的S1和S2蛋白。方法]利用不对称PCR方法以及采用设计酶切位点将2个片段连接的方法合成SARS香港株S蛋白上下游2个片段S1和S2;构建S1和S2这2个基因片段的原核表达载体,pET28a-S1和pET28a-S2,并转化BL21表达菌株,然后用IPTG诱导目的基因的表达并提取纯化目的蛋白。结果]连接得到S1和S2这2个基因片段,并成功地在大肠杆菌中得到表达。结论]该研究为后续SARS疫苗研究提供检测抗原奠定了基础。
|
| 关 键 词： | SARS S蛋白 S1蛋白 S2蛋白 原核表达 纯化 |
Synthesis,Expression and Purification of S1 and S2 Fragments of SARS S Protein in E.coli |
| |
| Institution: | ZHENG Shang-yong et al(Department of Pathogen biology,Second Millitary Medical University,PLA,Shanghai 200433) |
| |
| Abstract: | Objective] To obtain pure recombinant S1 and S2 of SARS S protein.Method] Using asymmetric PCR and ligation with endonuclease,S1 and S2 fragments of SARSV HK strain S gene were synthesized.Then,these two fragments were inserted into plasmid pET28a to obtain recombinant vectors pET28a-S1 and pET28a-S2,respectively.These recombinant vectors were transformed into E.coli BL21,and expression of S1 and S2 fragments were induced by IPTG.The conditions of expression and purification were optimized.Result] The S1 and S2 fragments were amplified and successfully expressed in E.coli.Conclusion] This research provides detection antigens for follow-up development of SARS vaccine. |
| |
| Keywords: | SARS S protein S1 protein S2 protein Prokaryotic expression Purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
