| 刺五加体细胞胚再生体系的研究 |
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| 引用本文： | 褚丽敏,赵洪利.刺五加体细胞胚再生体系的研究[J].中国农学通报,2010,26(10):263-266. |
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| 作者姓名： | 褚丽敏 赵洪利 |
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| 作者单位： | 1. 八五二农场第六管理区,黑龙江宝清,155626
2. 八五二农场第八管理区,黑龙江宝清,155626 |
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| 基金项目： | 辽宁省“十一五”攻关项目:设施农业高效生产技术集成与示范,辽宁省教育厅优秀人才项目 |
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| 摘 要： | 以刺五加种子为外植体，采用固体培养筛选出最优培养基，并成功地诱导出体细胞胚获得再生植株，以此来建立一套成熟稳定的刺五加体细胞胚再生体系。试验结果表明， 最适宜的胚性愈伤组织诱导培养基为1/3MS+1.0 mg?L-12,4-D,其诱导率可达50%；胚性愈伤组织增殖的最适培养基为1/3MS+1.0 mg?L-12,4-D，其增殖倍数可达4.24倍；胚性愈伤组织在不添加任何植物生长调节物质的1/3MS培养基上培养，可促进体细胞胚的形成、萌发及植株转化率。添加10 g?L-1蔗糖时，体细胞胚的发芽率达到90%，植株转化率达到97.8%。
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| 关 键 词： | 粮食总产量 粮食总产量 单产 播种面积 逐步回归 辽宁省 |
| 收稿时间： | 2009-12-16 |
| 修稿时间： | 2010-01-04 |
Study on regenerate system of Acanthopanax senticosus somatic embryo |
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| Chu Limin,Zhao Hongli.Study on regenerate system of Acanthopanax senticosus somatic embryo[J].Chinese Agricultural Science Bulletin,2010,26(10):263-266. |
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| Authors: | Chu Limin Zhao Hongli |
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| Institution: | 1The Sixth Management District of 852 Farm, Baoqing Heilongjiang 155626;
2The Eighth Management District of 852 Farm, Baoqing Heilongjiang 155626 |
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| Abstract: | Using seeds explants, an efficient plant regeneration system via somatic embryo was established for Acanthopanax senticosus . The results showed that 1/3 MS＋1.0 mg/L 2,4-D was the optimum medium for the embryogenic callus induction; 1/3 MS＋1.0 mg/L 2,4-D was the optimum medium for the embryogenic callus proliferation, propagating times was 4.24. It was increased the somatic embryo development that the embryogenic callus was transferred to 1/3 MS medium with 2,4-D lacking. Furthermore, when the medium was appended 10 g/L sucrose, the somatic embryo germination rate and plant conversion rate was 90% and 97.8% respectively. |
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| Keywords: | regenerate systemzz |
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