| 猪锚蛋白重复序列和SOCS盒蛋白基因4与生长因子受体结合蛋白基因10的克隆及印迹状态分析 |
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| 引用本文： | 周洋,朱江,焦明霞,王加强,孔庆然,刘忠华.猪锚蛋白重复序列和SOCS盒蛋白基因4与生长因子受体结合蛋白基因10的克隆及印迹状态分析[J].中国畜牧兽医,2013,40(8):1-7. |
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| 作者姓名： | 周洋 朱江 焦明霞 王加强 孔庆然 刘忠华 |
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| 作者单位： | 东北农业大学生命科学学院 |
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| 基金项目： | 国家自然科学基金(31101035);黑龙江省杰出青年基金(JC200905) |
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| 摘 要： | 本试验采用单核苷酸多态性(single nucleotide polymorphism,SNP)直接测序法检测猪锚蛋白重复序列和SOCS盒蛋白基因4(ankyrin repeat and SOCS box containing protein 4,Asb4)与生长因子受体结合蛋白基因10(growth factor recep-tor-bound protein 10,Grb10)在不同组织器官中的印迹状态。首先,克隆得到了1350bp的Asb4基因cDNA序列及1811bp的Grb10基因cDNA序列,然后进行了SNP直接测序法检测。结果发现,Asb4在1月龄仔猪所有检测组织器官中均为双等位基因表达,而Grb10在1月龄仔猪的舌、肾脏、胃、小肠和脑中为父源等位基因表达,在其他组织器官中为双等位基因表达。实时定量PCR结果表明,Grb10在10种组织器官中的表达量存在显著性差异(P<0.05),其中在肺脏组织中的表达量最高,在小肠和脑中表达量最低。上述结果表明,Grb10可能是猪父源表达的印迹基因,而Asb4则属于猪非印迹基因。
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| 关 键 词： | 猪 锚蛋白重复序列和SOCS盒蛋白基因4 生长因子受体结合蛋白基因10 单核苷酸多态性 印迹基因 |
| 收稿时间： | 2013-01-10 |
Cloning and Imprinting Analysis of Ankyrin Repeat and SOCS Box Containing Protein 4 and Growth Factor Receptor-bound Protein 10 in Swine |
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| ZHOU Yang,ZHU Jiang,JIAO Ming-xia,WANG Jia-qiang,KONG Qing-ran,LIU Zhong-hua.Cloning and Imprinting Analysis of Ankyrin Repeat and SOCS Box Containing Protein 4 and Growth Factor Receptor-bound Protein 10 in Swine[J].China Animal Husbandry & Veterinary Medicine,2013,40(8):1-7. |
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| Authors: | ZHOU Yang ZHU Jiang JIAO Ming-xia WANG Jia-qiang KONG Qing-ran LIU Zhong-hua |
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| Institution: | College of Life Science, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | In this study, by using a single nucleotide polymorphism-based method, 1350 bp cDNA sequence of porcine ankyrin repeat and SOCS box containing protein 4 (Asb4) and 1811 bp cDNA sequence of porcine growth factor receptor-bound protein 10 (Grb10) was obtained. Then we identified their imprinting status in ten tissues. Imprinting analysis showed that both alleles of Asb4 were expressed in all the tested tissues. Grb10 was paternally expressed in tongue, kidney, stomach, intestine and brain, while biallelically expressed in the others. We also used quantitative Real-time PCR to assess the expression of Grb10 in these tissues. The expression level of Grb10 was significantly different among tissues, which was the highest in lung and lowest in both small intestine and brain. These results primarily indicated that Grb10 probably was a porcine paternally expressed gene, but Asb4 was not imprinted in swine. |
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| Keywords: | pig ankyrin repeat and SOCS box containing protein 4 growth factor receptor-bound protein 10 single nucleotide polymorphism imprinting gene |
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