| 苹果果实酸/低酸性状的SSR分析 |
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| 引用本文： | 姚玉新,翟衡,赵玲玲,伊凯,刘志,宋烨.苹果果实酸/低酸性状的SSR分析[J].园艺学报,2006,33(2):244-246. |
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| 作者姓名： | 姚玉新 翟衡 赵玲玲 伊凯 刘志 宋烨 |
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| 作者单位： | (山东农业大学园艺科学与工程学院, 泰安271018; 辽宁省果树科学研究所, 熊岳115214) |
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| 基金项目： | 山东省农业良种产业化工程项目 |
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| 摘 要： | 以91株‘东光’和‘富士’的正反交F1 代群体为试材, 测定了成熟果实可滴定酸含量和果实不同发育阶段苹果酸含量的变化, 从表型上分析了苹果酸的遗传规律。利用140对共显性遗传的SSR引物对高酸和低酸个体群体分离分析, 筛选到一个与果实酸/低酸性状连锁的标记SDY085, 遗传距离为8.89 cM, 表型分析和标记分析都表明苹果果实酸/低酸性状由一对主效基因Ma/ma控制, 并且Ma对ma为完全显性, 而酸个体中苹果酸的连续分布则是加性多基因作用的结果。
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| 关 键 词： | 苹果 酸/低酸性状 SSR标记 苹果酸 |
| 文章编号： | 0513-353X（2006）02-0244-05 |
| 收稿时间： | 2005-5-9 |
| 修稿时间： | 2005年5月9日 |
Analysis of Apple Fruit Acid/Low-acid Trait by SSR Marker |
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| Yao Yuxin,Zhai Heng,Zhao Lingling,Yi Kai,Liu Zhi,Song Ye.Analysis of Apple Fruit Acid/Low-acid Trait by SSR Marker[J].Acta Horticulturae Sinica,2006,33(2):244-246. |
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| Authors: | Yao Yuxin Zhai Heng Zhao Lingling Yi Kai Liu Zhi Song Ye |
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| Institution: | 1.The Center for Infectious Disease, 458 Hospital, Guangzhou 510602, China;2.The Ningbo Hospital for Infectious Disease, Ningbo 315010, China |
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| Abstract: | It is necessary for app le genomic research and breeding to find outmalic acid genetic characteristics. In this research, SSR marker linked to acid /low-acid trait in app le fruitwas identified from 140 SSR primer pairs, materialed by 91 F1 population hybrid from the intraspecific cross between apple cultivar‘Dongguang’ and ‘Fuji’. Of 140 SSR primer pairs, only p rimer SDY085 produced a polymorphic band linked to acid trait, and the linkage distance was 8.89 cM. Also, the titrated acid and malic acid in different developmental stages were determined. The SSR marker analysis, coupled with the change of the total acid and malic acid contents, revealed that acid/low-acid trait was governed by a major gene and acid trait was complete dominant. |
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| Keywords: | Apple Malus ×domestica Borkh Malus ×domestica Borkh Acid/low-acid trait SSR marker Malic acid |
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