Assessment of the uniformity of wheat and tomato varieties at DNA microsatellite loci |
| |
| Authors: | RJ Cooke GMM Bredemeijer MW Ganal R Peeters P Isaac S Rendell J Jackson MS Röder V Korzun K Wendehake T Areshchenkova M Dijcks D Laborie L Bertrand B Vosman |
| |
| Institution: | (1) NIAB, Huntingdon Road, Cambridge, CB3 0LE, U.K.;(2) Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands;(3) Institute for Plant Genetics and Crop Plant Research (IPK), Corenstrasse 3, D-06466 Gatersleben, Germany;(4) Present address: TraitGenetics GmbH, D-06466 Gatersleben, Germany;(5) Nunhems Zaden B.V., P.O. Box 4005, 6080 AA Haelen, The Netherlands;(6) Agrogene SA, 620 ave. Blaise Pascal, Z.I., 77550 Moissy Cramayel, France;(7) Present address: CTRC-IDD, San Antonio, Texas, Tx, 78245, U.S.A |
| |
| Abstract: | By analysing a number (20–38) of individuals from selected varieties of wheat and tomato, we have been able to assess intra-varietal
uniformity at certain micro satellite (simple sequence repeat, SSR) loci. In total, 45 varieties of wheat were analysed at
between 7–9 different SSR loci, and 10 varieties of tomato were analysed at six loci. The results showed that there was variation
both between varieties and between microsatellites in the degree of non-uniformity observed, and it was possible to identify
a number of different probable sources of non-uniformity. Twenty-four of the wheat varieties and nine of the tomato varieties
were sufficiently uniform to meet the standards currently applied for distinctness, uniformity and stability (DUS) testing
using phenotypic characteristics. The implications for the potential future use of SSRs in DUS testing are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
| Keywords: | microsatellites SSR tomato uniformity variety testing wheat |
| 本文献已被 SpringerLink 等数据库收录！ |
|
