| TISSUE CULTURE OF ROSA CHINENSIS VAR.FLORIBUNDA |
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| 作者姓名： | 朱虹 |
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| 作者单位： | Northeast Forestry University，Harbin 150040. China |
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| 摘 要： | INTRODUCTI0NScIllossManl1hcin1-t"hicl1isbclongtoRosaccacan`lRo.vol.'I1asbeautifulcolorandgoodt1oxtcrshapcltsflottcrlngpcrlod1sverylong(2~3mol1tl1s)SchlossMannhclmcanli"cundcrdiffcrcntcli1natcc0nditionsandcanbccultlX'atcdinpotandpla11tcdal0ngstrccts.Thctissueculturcn1ethod\"astlscdinthISstudy'f0rincrcasingpropagationratcofSclllossMannhcin1.MATERIALSANDMETHO0SExPcrimcntaIMatcriaIsScl1IossMa11nI1cll111\asscIcctcdIYon1grccnhousclnAugustof1`j()4Fulla11dunsproutll1gbudsxtcrctakc…
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TISSUE CULTURE OF ROSA CHINENSIS VAR. FLORIBUNA |
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| Zhu Hong.TISSUE CULTURE OF ROSA CHINENSIS VAR.FLORIBUNDA[J].Journal of Forestry Research,1996(2). |
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| Authors: | Zhu Hong |
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| Abstract: | The tissue cullurc of Schloss Mannheim (Rosa Chinensus , var. Florihunda ) with full and unsprouting bud of stem segments as the explants was experimented The result shows that the buds sprouted best on MS medium with the addition of 6-HA 1 .0 mg/L,. and differentiation was best on MS medium with addition of 6-BA 1 .5 NAA0.05 ZT0.1 mg/L, or KT 1,0 NAA0,05 ZT0.1mg/L. The MS emdium with addition of 6-BA 0.3 NAA 0.0 5 ZT0.1 mg/L, or KT 0.3 NAA0.05 ZT0. 1 mg/L showed a good result for developing strong shoots. 1/2 MS medium with the addition ofIBA 0.1 mg/L, or IBA0.1 NAA0.02 mg/L, had best result for rooting. The plantlets should be transplanted from test-tube to soil when they grew ' to 2.5 ~ 4.0 cm high and have 3 ~ 5 strips short roots. Ahigher survival rate was obtained under the conditions of conterolling humidity and temperature |
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| Keywords: | Rosa Chinensis var Floribund Tissue culture Cytokinin Auxin |
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