| 贝莱斯芽胞杆菌HN-2发酵上清液抑制三种黄单胞菌的生物活性研究 |
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| 引用本文： | 谭峥,王雨,杨欢,刘文波,缪卫国,靳鹏飞.贝莱斯芽胞杆菌HN-2发酵上清液抑制三种黄单胞菌的生物活性研究[J].中国生物防治学报,2020,36(3):414-420. |
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| 作者姓名： | 谭峥 王雨 杨欢 刘文波 缪卫国 靳鹏飞 |
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| 作者单位： | 海南大学植物保护学院/热带农林生物灾害绿色防控教育部重点实验室, 海口 570228 |
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| 基金项目： | 海南省重点研发计划项目（ZDYF2018240）；国家重点研发计划（2018YFD0201105）；国家自然科学基金（31960552）；海南省科协青年科技英才学术创新计划项目（QCXM201903）；海南大学校内经费（KYQD（ZR）1842）；海南自然科学基金创新研究团队项目（2016CXTD002） |
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| 摘 要： | 贝莱斯芽胞杆菌Bacillus velezensis HN-2为本实验室从土壤中分离得到的一株生防菌，本文研究了菌株HN-2发酵上清液活性成分的最优提取方式、抑菌效果及其活性成分的稳定性。结果表明，通过不同方式提取的菌株HN-2发酵上清液活性成分对3种黄单胞菌均有良好的抑菌活性。其中正丁醇提取物对水稻细菌性条斑病菌Xanthomonas oryzae pv. oryzicola的抑菌效果最好，抑菌圈大小为（41.57±0.33）mm；并且在10 mg/mL浓度下，贝莱斯芽胞杆菌HN-2正丁醇提取物比杆菌肽、春雷霉素和叶枯唑具有更好的抑菌效果。稳定性试验结果表明，贝莱斯芽胞杆菌HN-2正丁醇提取物对pH有较宽的适应范围（pH 1.0～9.0），且在紫外线及高温等环境下仍具有较好的稳定性。综上所述，贝莱斯芽胞杆菌HN-2正丁醇提取物具有开发成为生物源农药的潜力。
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| 关 键 词： | 贝莱斯芽胞杆菌 黄单胞菌 抑菌活性 稳定性 正丁醇提取物 |
| 收稿时间： | 2019-09-05 |
Inhibition of Bacillus velezensis HN-2 Fermentation Supernatant on Three Kinds of Xanthomonas |
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| TAN Zheng,WANG Yu,YANG Huan,LIU Wenbo,MIAO Weiguo,JIN Pengfei.Inhibition of Bacillus velezensis HN-2 Fermentation Supernatant on Three Kinds of Xanthomonas[J].Chinese Journal of Biological Control,2020,36(3):414-420. |
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| Authors: | TAN Zheng WANG Yu YANG Huan LIU Wenbo MIAO Weiguo JIN Pengfei |
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| Institution: | College of Plant Protection, Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests(Hainan University), Ministry of Education, Haikou 570228, China |
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| Abstract: | Bacillus velezensis strain HN-2 is a biocontrol bacterial strain isolated from soil in our laboratory. In this study, we investigated the optimal extraction methods, inhibition activity and stability of active components in the fermentation supernatant of strain HN-2. The results showed thatn-butyl alcohol extract, ammonium sulfate precipitation extract and acidification extract of B. velezensis HN-2 exhibited potent inhibitory activity against three kinds of Xanthomonas. The n-butyl alcohol extract had the strongest activity against Xanthomonas oryzae pv. oryzicola with the inhibition zone of (41.57±0.33) mm. Compared with bacitracin, kasugamycin and bismerthiazol, the n-butyl alcohol extract at 10 mg/mL had higher antibacterial activity. Stability experiment showed that the active substance of n-butyl alcohol extract had a broad adaption range to pH value (pH 1.0-9.0), and was stable under high temperatures and UV-treatment. In conclusion, the results revealed that the n-butyl alcohol extract of strain HN-2 with strong antibacterial activity and stability had the potential to be developed into biocontrol agent. |
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| Keywords: | Bacillus velezensis Xanthomonas antibacterial activity stability n-butyl alcohol extract |
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