| 解淀粉芽胞杆菌XJ5挥发性物质抑菌活性及对苹果褐腐病防效测定 |
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| 引用本文： | 郭洁心,张育铭,朱洪磊,任璐,张宝俊.解淀粉芽胞杆菌XJ5挥发性物质抑菌活性及对苹果褐腐病防效测定[J].中国生物防治学报,2020,36(4):575-580. |
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| 作者姓名： | 郭洁心 张育铭 朱洪磊 任璐 张宝俊 |
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| 作者单位： | 山西农业大学植物保护学院, 太谷 030801 |
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| 基金项目： | 山西省科技基础条件平台项目（201605D121024）；山西省应用基础研究计划（201801D121244） |
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| 摘 要： | 解淀粉芽胞杆菌Bacillus amyloliquefaciens XJ5挥发性物质对苹果褐腐病菌Monilinia fructigena等多种病原真菌具有良好的拮抗活性。通过固相微萃取-气相色谱质谱联用仪（Solid-phase microextraction-Gas chromatography mass spectrometry，SPME-GC-MS）对菌株XJ5产生的挥发性物质进行检测，利用平板对扣法测定挥发性化合物的抑菌活性，并离体接种评价菌株XJ5挥发性物质对苹果褐腐病的防效。结果表明，XJ5菌株挥发性物质对9种植物病原真菌的抑菌率在47.9%~84.8%，其中对苹果褐腐病菌的抑菌率最高为84.8%。SPME-GC-MS检测及抑菌活性测定表明，菌株XJ5分泌的主要挥发性物质为十二醛（Dodecanal），具有抑菌活性的挥发性物质主要为2-壬醇（2-Nonanol）、2-乙基己醇（2-Ethyl-1-hexanol）和2-十一醇（2-Undecanol）。其中2-壬醇抑制苹果褐腐病菌的EC50为3.43 μg/mL，且XJ5菌液离体熏蒸与2-壬醇离体熏蒸均可有效抑制苹果褐腐病菌丝生长及病斑扩展，其对苹果褐腐病的离体防效分别在14.4%~71.2%和66.4%~97.6%，与对照差异显著。
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| 关 键 词： | 解淀粉芽胞杆菌 挥发性物质 SPME-GC-MS 抑菌活性 离体防效 |
| 收稿时间： | 2020-02-05 |
Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena |
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| GUO Jiexin,ZHANG Yuming,ZHU Honglei,REN Lu,ZHANG Baojun.Antifungal Activity of Volatile Organic Compounds from Bacillus amyloliquefaciens XJ5 and Control Effect against Monilinia fructigena[J].Chinese Journal of Biological Control,2020,36(4):575-580. |
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| Authors: | GUO Jiexin ZHANG Yuming ZHU Honglei REN Lu ZHANG Baojun |
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| Institution: | College of Agronomy, Shanxi Agricultural University, Taigu 030801, China |
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| Abstract: | Volatile organic compounds (VOCs) of Bacillus amyloliquefaciens XJ5 have good antifungal activity against various pathogenic fungi such as Monilinia fructigena. The VOCs produced by strain XJ5 were detected by SPME-GC-MS, their antifungal activities were determined by two-sealed-base-plates method, and the control effects against M. fructigena were evaluated in vitro. The results indicated that inhibition rate of XJ5 VOCs against nine pathogenic fungi were ranged between 47.9% and 84.8%, and the highest inhibition rate against M. fructigena was 84.8%. SPME-GC-MS analysis and antifungal activity determination showed that the major VOCs produced by strain XJ5 were Dodecanal, and the major VOCs with antifungal activity were 2-Nonanol, 2-Ethyl-1-hexanol and 2-Undecanol. The EC50 value of 2-Nonanol inhibiting M. fructigena was 3.43 μg/mL. In vitro fumigation with XJ5 bacteria suspension and 2-Nonanol can effectively inhibited the mycelial growth of M. fructigena and the lesion development. The fumigation control efficacies of XJ5 bacteria suspension and 2-Nonanol against M. fructigena were 14.4%~71.2% and 66.4%~97.6%, respectively, significantly different from those of the control. |
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| Keywords: | Bacillus amyloliquefaciens volatile organic compounds SPME-GC-MS antifungal activity control effectin in vitro |
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