| 绵羊肺源致病性大肠杆菌的分离鉴定及耐药性研究 |
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| 引用本文： | 郭强强,杨彩虹,柴迎锦,顾晓晓,钟发刚,黄新,张星星,吴桐忠,李劼,韩猛立,周霞.绵羊肺源致病性大肠杆菌的分离鉴定及耐药性研究[J].中国动物传染病学报,2020(3):29-34. |
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| 作者姓名： | 郭强强 杨彩虹 柴迎锦 顾晓晓 钟发刚 黄新 张星星 吴桐忠 李劼 韩猛立 周霞 |
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| 作者单位： | 新疆农垦科学院省部共建绵羊遗传改良与健康养殖国家重点实验室;武威市工业企业人才交流开发服务中心;石河子大学动物科技学院 |
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| 基金项目： | 省部共建绵羊遗传改良与健康养殖国家重点实验室优秀中青年人才培养引导计划专项资助(SKLSGIHP2016A02,MYSKLKF201804,SKLSGIHP2017A03);兵团重大科技项目计划(2017AA006-04);兵团现代农业科技攻关与成果转化计划项目(2016AC018);兵团区域创新引导计划(2018BB036,2017BA038). |
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| 摘 要： | 为了查清新疆石河子部分规模场引起羔羊死亡的原因,本研究对采集的病羊肺脏组织进行细菌分离,同时对分离菌株进行小鼠致病性试验、特异性基因PCR检测、药物敏感性试验和耐药基因PCR检测.结果显示:从病变肺脏组织分离得到10株细菌,分离菌为大肠杆菌,致病性强;分离菌株呈现多重耐药现象,对诺氟沙星、复方新诺明、氟苯尼考等17种抗生素耐药;检测到iutA、fyuA、ireA、hlyD和afa 5种毒力因子,strA、strB、aadA1/aadA2、Bla(TEM1)、Bla(OXA1)、Tet A和Tet B 7种耐药基因.本研究为该地区规模场绵羊感染大肠杆菌的防控和临床用药提供依据.
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| 关 键 词： | 绵羊 肠外致病性大肠杆菌 毒力因子 耐药基因 药敏试验 |
ISOLATION,IDENTIFICATION AND DRUG RESISTANCE OF PATHOGENIC ESCHERICHIA COLI STRAINS FROM DIEASED SHEEP LUNGS |
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| GUO Qiang-qiang,YANG Cai-hong,CHAI Ying-jin,GU Xiao-xiao,ZHONG Fa-gang,HUANG Xin,ZHANG Xing-xing,WU Tong-zhong,LI Jie,HAN Meng-li,ZHOU Xia.ISOLATION,IDENTIFICATION AND DRUG RESISTANCE OF PATHOGENIC ESCHERICHIA COLI STRAINS FROM DIEASED SHEEP LUNGS[J].Chinese Journal of Animal Infectious Diseases,2020(3):29-34. |
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| Authors: | GUO Qiang-qiang YANG Cai-hong CHAI Ying-jin GU Xiao-xiao ZHONG Fa-gang HUANG Xin ZHANG Xing-xing WU Tong-zhong LI Jie HAN Meng-li ZHOU Xia |
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| Institution: | (Xinjiang Academy of Agricultural and Reclamation Science/Skate key Laboratory for Sheep Genetic Improvement and Healthy Production,Shihezi 832000,China;Wuwei Industrial Enterprise Talent Exchange and Development Service Center,Wuwei 733000,China;College of Animal Science and Technology,Shihezi University,Shihezi 832003,China) |
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| Abstract: | In order to determine the etiology of deaths of sheep suffered from respiratory disease in Shihezi,Xinjiang,the bacterial isolation and identification were performed and 10 E.coli strains were obtained from lung tissues.These strains were highly pathogenic when inoculated into mice.The drug testing found that these strains were resistant to 17 antibiotics,such as norfloxacin,cotrimoxazole and florfenicol.In addition,5 virulence factors(iutA,fyuA,ireA,hlyD and afa 5)and 7 drug resistance genes(strA,strB,aadA1/aadA2,Bla(TEM1),Bla(OXA1),Tet A and Tet B)were detected from these strains by PCR.The result provided the reference for the prevention and control of E.coli respiratory infection in sheep. |
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| Keywords: | Sheep extraintestinal pathogenic Escherichia coli virulence gene drug resistance gene drug susceptibility |
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