| 橡胶草胶乳蛋白双向电泳方法的建立 |
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| 引用本文： | 黄荣辉,校现周,仇键,刘实忠,罗世巧,王启超,梅志刚,牛静明.橡胶草胶乳蛋白双向电泳方法的建立[J].热带作物学报,2013,34(2):272-275. |
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| 作者姓名： | 黄荣辉 校现周 仇键 刘实忠 罗世巧 王启超 梅志刚 牛静明 |
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| 作者单位： | 1. 海南大学园艺园林学院,海南海口 570228;中国热带农业科学院橡胶研究所,海南儋州 571737
2. 中国热带农业科学院橡胶研究所,海南儋州,571737 |
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| 基金项目： | 乙烯刺激对橡胶树萜类次生代谢的调控（No. XJSYWFZX2009-15）。 |
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| 摘 要： | 采用Tris饱和酚法和TCA/丙酮沉淀法提取橡胶草(Taraxacum Kok-saghyz Rodin)胶乳蛋白质,并建立蛋白双向电泳方法.结果表明,TCA/丙酮沉淀法得到的双向电泳图谱横条纹较多,背景模糊,得到约446个蛋白质点；Tris饱和酚法提取样品双向电泳的聚焦效果较好,图谱显示约630个蛋白点,其中包括较丰富的低分子量蛋白.
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| 关 键 词： | 橡胶草 胶乳 蛋白质 电泳 |
Establishment of Two-dimensional Electrophoresis for TaraxacumKok-saghyz Rodin Latex Protein |
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| HUANG Ronghui,XIAO Xianzhou,QIU Jian,LIU Shizhong,LUO Shiqiao,WANG Qichao,MEI Zhigang and NIU Jingming.Establishment of Two-dimensional Electrophoresis for TaraxacumKok-saghyz Rodin Latex Protein[J].Chinese Journal of Tropical Crops,2013,34(2):272-275. |
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| Authors: | HUANG Ronghui XIAO Xianzhou QIU Jian LIU Shizhong LUO Shiqiao WANG Qichao MEI Zhigang and NIU Jingming |
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| Institution: | 1 College of Horticulture and Landscape Architecture, Hainan University
2 Rubber Research Institute, CATAS;Rubber Research Institute, CATAS;Rubber Research Institute, CATAS;Rubber Research Institute, CATAS;Rubber Research Institute, CATAS;1 College of Horticulture and Landscape Architecture, Hainan University
2 Rubber Research Institute, CATAS;1 College of Horticulture and Landscape Architecture, Hainan University
2 Rubber Research Institute, CATAS;1 College of Horticulture and Landscape Architecture, Hainan University
2 Rubber Research Institute, CATAS |
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| Abstract: | |
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| Keywords: | Taraxacum Kok-saghyz Rodin Latex Protein Electrophoresis |
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