| 黄粉虫不同生长阶段诱导抗菌肽的效果分析 |
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| 引用本文： | 王立新,王姗姗,戴四发,路振香,王金虎,胡 静静.黄粉虫不同生长阶段诱导抗菌肽的效果分析[J].中国农学通报,2009,25(5):10-13. |
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| 作者姓名： | 王立新 王姗姗 戴四发 路振香 王金虎 胡 静静 |
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| 作者单位： | 安徽科技学院动物科学学院,安徽风阳,233100 |
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| 基金项目： | 安徽省高校自然科学基金重点项目 |
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| 摘 要： | 摘 要：目的 分析黄粉虫不同生长阶段所提取的抗菌肽抗菌活性及抗菌肽组分差异。方法 通过针刺使黄粉虫各虫态感染大肠杆菌产生抗菌肽，经过研磨、离心等步骤提取抗菌肽，用药敏试验检测这些抗菌肽的抗菌效果，并用聚丙烯酰胺凝胶电泳试验分离其蛋白及多肽条带。结果 药敏试验的结果表明处于蛹期和成虫期的黄粉虫所产生抗菌肽的抑菌效果不如处于幼虫期所产生抗菌肽的抑菌效果明显，但差异不显著；电泳图谱显示黄粉虫幼虫有8条带，成虫有5条，蛹有5条，说明黄粉虫蛹期和成虫的抗菌肽种类较幼虫期的少，但是它们都还有更多的小肽段未被分离开。
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| 关 键 词： | 农业科技人才 农业科技人才 队伍建设 政策研究 |
| 收稿时间： | 2008-11-10 |
| 修稿时间： | 2009-01-12 |
Analysis on Antibacterial Activity and Anti-Peptide of Different Growth Stages from Tenebrio Molitor |
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| Wang Lixin,Wang Shanshan,Dai Sif,Lu Zhenxiang,Wang Jinhu,Hu Jing.Analysis on Antibacterial Activity and Anti-Peptide of Different Growth Stages from Tenebrio Molitor[J].Chinese Agricultural Science Bulletin,2009,25(5):10-13. |
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| Authors: | Wang Lixin Wang Shanshan Dai Sif Lu Zhenxiang Wang Jinhu Hu Jing |
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| Institution: | (Department of Animal Science, Anhui Technology College, Fengyang Anhui 233100) |
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| Abstract: | Abstract: Objective The purpose of the study was to analyze the cecropin antibacterial activity and different component of antibacterial peptide in different growth stages. Material and Methods Tenebrio Molitor produced antimicrobial peptides in different stages, after infected E. coli with the method of acupuncture. Antimicrobial peptides were obtained after grinding and centrifugating the infected Tenebrio Molitor, and determined antimicrobial effects of these antimicrobial peptides with drug-test. Protein and polypeptide were separated with the method of polyacrylamide. Results The results of the sensitivity test showed that bacteriostasis effect of antibacterial peptide of Tenebrio Molitor in the larval stage was more effect than that of antibacterial peptide of Tenebrio Molitor in the pupa and the adult phase, but there was no significant difference. Tenebrio Molitor electrophorogram showed that there were 8 genophore in larvae stage, 5 genophore in prosopon, and 5 genophore in pupa. The result showed that the variety of antimicrobial peptides of Tenebrio Molitor in pupa and adult was less than that of in larvae, however, more little peptide were not separated from. Tenebrio Molito of each stage. |
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| Keywords: | Tenebrio molitor induction Antibacterial Peptide |
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