SSR fingerprinting Chinese peach cultivars and landraces (Prunus persica) and analysis of their genetic relationships |
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| Authors: | Zhongping Cheng Hongwen Huang |
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| Institution: | 1. Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, Hubei 430074, P.R. China;2. Key Laboratory for Protection of Huazhong Plant Resources and Sustainable Utility, Wuhan, Hubei 430074, P.R. China;3. South China Botanical Garden, Chinese Academy of Sciencs, Guangzhou, Guangdong 510650, P.R. China |
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| Abstract: | Thirty-two Chinese peach landraces/cultivars, a major subset of the core Chinese peach collection, were fingerprinted using seven pairs of SSR primers to assess their genetic diversity and relatedness. The seven primer pairs detected eight loci and revealed an allele richness of 3.125 (average alleles per locus), an expected heterozygosity (He) of 0.450, and a Shannon index of 0.728 among the landraces/cultivars. This level of genetic diversity is lower compared to other fruit trees and Prunus congenus species (cherry and apricot), but it is comparable to previous reports in peaches. A greater level of genetic diversity was observed in landraces than in cultivars, indicating that peach landraces are valuable for germplasm collection. All cultivars and landraces, except two, were unambiguously identified based on multi-locus genotypes. Eight unique alleles were detected among this group of Chinese peaches. UPGMA clustering analysis separated the 32 cultivars/landraces into two distinct groups, which is generally in accordance with the known pedigree information. The results provide accurate genetic information for defined acquisition policy in the repositories, improving the integrity and efficiency of germplasm management and giving evidences for protection of breeder's intellectual rights. |
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| Keywords: | SSR simple sequence repeat UPGMA un-weighted pair group method with arithmetic average CTAB hexadecyltrimethyl ammonium bromide PVP polvinylpyrrolidone |
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