Multiple shoot regeneration in seed-derived immature leaflet explants of peanut (Arachis hypogaea L.) |
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| Authors: | Siddharth Tiwari Rakesh Tuli |
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| Institution: | Plant Molecular Biology and Genetic Engineering Division, National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow 226001, U.P., India |
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| Abstract: | A protocol was developed for organogenesis from immature leaflet explants derived from mature seeds of peanut. Immature leaflets pre-incubated on MS medium supplemented with 13.32 μM BAP + 4.95 μM NAA for 7 days, turned green and enlarged. The enlarged green leaflets produced multiple shoot buds after 1–2 cycles of sub-culture on MS medium supplemented with 13.32 μM BAP. Three cycles of shoot buds on the elongation medium (13.32 μM BAP) produced 6.17 ± 0.47 elongated shoots per explant. The shoot bud formation was genotype independent. All elongated shoots rooted on the medium containing 4.95 μM NAA. The complete protocol gave efficient (>81%) direct organogenesis, leading to the development of plantlets within 4 months. |
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| Keywords: | BAP 6-benzylaminopurine MS Murashige and Skoog (1962) medium NAA α-naphthalene acetic acid |
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