| 樱桃谷鸭白介素18基因的克隆与原核表达 |
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| 引用本文： | 龚朋飞,周庆丰,苏润环,周美华,郑志青.樱桃谷鸭白介素18基因的克隆与原核表达[J].广东畜牧兽医科技,2009,34(2):28-30. |
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| 作者姓名： | 龚朋飞 周庆丰 苏润环 周美华 郑志青 |
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| 作者单位： | 广东温氏食品集团研究院,广东,云浮,527400 |
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| 摘 要： | 从鸡新城疫病毒刺激的鸭脾脏淋巴细胞培养液中提取基因组RNA，采用RT—PCR方法扩增鸭IL-18基因，插入pMD18-T载体，进行序列测定与分析。将成熟鸭IL-18基因亚克隆到表达载体PBCX中，并转化入大肠杆菌E．coliBL21进行表达。测序结果表明，该基因由603个核苷酸组成，包含一个完整阅读框，共编码201个氨基酸。表达载体经IPTG诱导表达出相对分子量约为59KD的融合蛋白。
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| 关 键 词： | 鸭白介素1 8(DuIL-18) 基因克隆 原核表达 |
Cloning ofInterleukin-18 Mature Protein Gene of Cherry Valley Duck and its Exoression in Eschenctna coil |
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| GONG Pengfei,ZHOU Qingfeng,SU Runhuan,ZHOU Meihua,ZHENG Zhiqing.Cloning ofInterleukin-18 Mature Protein Gene of Cherry Valley Duck and its Exoression in Eschenctna coil[J].Guangdong Journal of Animal and Veterinary Science,2009,34(2):28-30. |
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| Authors: | GONG Pengfei ZHOU Qingfeng SU Runhuan ZHOU Meihua ZHENG Zhiqing |
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| Institution: | (Research Institute of GuangDong WENS Food Group, YunFu, Guangdong 527400, China) |
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| Abstract: | Duck Interleukin-18 （DuIL-18） gene was amplified from chicken NDV-stimulated spleen lymphocytes by the method of RT-PCR The PCR product was cloned into the pMDI 8-T vector for sequencing and nucleic acid analysis. A prokaryotic expression plasmid, pBCX -ChiL18 was obtained by subcloning the encoding region of the DuIL-18 mature peptide into pBCX. Sequencing analysis showed that the nucleotide sequence of this DuIL-18 mature protein gene was 603bp long, including a complete ORF which encoded 201 amino acids. The recombinant DuIL- 18 gene was expressed efficiently in pBCX-DuIL- 18 plasmid transformed Escherichia coli strain （BL21） after induced by IPTG, the relative molecular weight ofthe mature peptide protein was approximately 59KD. |
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| Keywords: | Duck interleukin- 18 （DuIL- 18） Gene cloning Prokaryotic expression |
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