| 玉米DCL1基因鉴定及其自然等位变异分析 |
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| 引用本文： | 徐东东,赵永平,王建海,周强,谢传晓.玉米DCL1基因鉴定及其自然等位变异分析[J].玉米科学,2015,23(1):17-25. |
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| 作者姓名： | 徐东东 赵永平 王建海 周强 谢传晓 |
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| 作者单位： | 中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081;中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081;中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081;四川农业大学, 成都 611130;中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081;安徽农业大学, 合肥 230031;中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京 100081 |
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| 基金项目： | 国家自然科学基金面上项目(No.31171562) |
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| 摘 要： | 根据生物信息学分析,同源性克隆分离玉米DCL1候选基因,鉴定该基因组织与逆境响应等表达特征,并分析其自然等位变异。结果表明,目标候选基因全长7 408 bp,与拟南芥和水稻DCL1基因氨基酸序列高度保守,相似度分别为91%和77%。mRNA定量PCR分析表明,该基因在幼叶和吐丝期雌穗中表达丰度分别是幼茎的75.35倍和16.21倍;高盐、脱水、ABA、低氮、低磷胁迫处理条件下,幼苗中高盐和ABA处理呈下调表达,低氮和低磷处理呈显著上调表达,脱水处理无显著变化。核苷酸与氨基酸序列的保守性、组织与逆境响应差异表达特征均与拟南芥和水稻等相似。87份玉米自交系10×全基因重测序数据分析结果表明,该基因遗传变异丰富,共83个位点存在自然变异,但外显子区域和主要的功能结构域序列相对保守,约2/3的自然变异集中在内含子区域,外显子区域的27个自然变异位点只有8个位点在16个材料存在氨基酸差异。
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| 关 键 词： | 玉米 DCL1基因 基因结构 功能域 重测序 自然等位变异 |
| 收稿时间： | 2014/2/25 0:00:00 |
Identification of Maize DCL1 Gene and Analysis of Its'Natural Alleles |
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| XU Dong-dong,ZHAO Yong-ping,WANG Jian-hai,ZHOU Qiang and XIE Chuan-xiao.Identification of Maize DCL1 Gene and Analysis of Its''Natural Alleles[J].Journal of Maize Sciences,2015,23(1):17-25. |
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| Authors: | XU Dong-dong ZHAO Yong-ping WANG Jian-hai ZHOU Qiang and XIE Chuan-xiao |
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| Institution: | Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081;Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081;Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081;Sichuan Agricultural University, Chengdu 611130;Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081;Institute of Crop Science, Chinese Academy of Agricultural Sciences, National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081 |
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| Abstract: | The candidate gene for maize DCL1was isolated based on bioinformatics analysis and characterized its expression across the different tissues of maize and in response to various abiotic stresses via real-time quantitative PCR(qRT-PCR) and identified its natural variation based on resequencing among the diverse lines. The maize DCL1candidate gene was 7 408 bp. The amino acid sequence of it was 91% and 77% conserved with Arabidopsis and rice DCL1protein, respectively. QRT-PCR results indicated ZmDCL1gene expressed in leaves at seedling stage and ears at silking stage were respectively 75.35 times and 16.21 times relative to the seedling shoots. It was up-regulated under low nitrogen or low phosphorus and down-regulated under NaCl or ABA. Based on amino acid and nucleotide sequence conservatism, expression profiles across different tissues and response to the different abiotic stresses. The natural variation of this gene was analyzed by using the whole genomes 10×resequencing data across 87 maize diverse lines.It indicated that there were 83 SNPs variation within ZmDCL1among these lines. However, it was highly conservative in coding regions especially the functional domains. About two-thirds of the SNPs existed in introns, while 8 of 27 SNPs in exon region altered the amino acid. |
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| Keywords: | Maize DCL1 Gene structure Functional domain Resequencing Natural allele |
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