| ‘异株’荨麻组织培养技术体系的建立 |
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| 引用本文： | 李江舟,庄杰,金晓彤,朱然,张晋,卓馨怡,侯义龙.‘异株’荨麻组织培养技术体系的建立[J].分子植物育种,2020(7):2331-2337. |
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| 作者姓名： | 李江舟 庄杰 金晓彤 朱然 张晋 卓馨怡 侯义龙 |
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| 作者单位： | 大连大学生命科学与技术学院 |
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| 基金项目： | 辽宁省自然科学基金指导性计划项目(20170540035);大连市科技计划项目(2015E21SF014);辽宁省大学生创新创业训练计划项目共同资助。 |
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| 摘 要： | ‘异株’荨麻是一种一年生或多年生的草本植物,既有食用价值,也具药用价值,研究‘异株’荨麻组织培养快繁技术体系很有意义。本研究以‘异株’荨麻的嫩梢茎段为外植体,采用次氯酸钠配合乙醇灭菌技术对异株荨麻的嫩梢茎段进行灭菌,在对异株荨麻外植体的初代培养、增殖培养和生根培养条件进行研究的基础上,建立了‘异株’荨麻快速繁殖的组织培养技术体系。该体系为:2%次氯酸钠5 min+75%乙醇0.5 min为最适灭菌方法;最适初代培养基为MS+0.25 mg/L 6-BA+0.25 mg/L IBA+0.25 mg/L KT+0.25 mg/L反-ZT+0.7%琼脂+3%蔗糖;最适继代增殖培养基为2/3MS+0.5 mg/L 6-BA+0.3 mg/L IBA+0.25 mg/L反-ZT+0.7%琼脂+3%蔗糖;最适生根培养基为MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.7%琼脂+3%蔗糖。
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| 关 键 词： | 异株荨麻(Urtica dioica) 嫩梢茎段 组织培养 |
Establishment of Plant Tissue Cultural Technical System in Nettle Cultivar ’Yizhu’ |
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| Li Jiangzhou,Zhuang Jie,Jin Xiaotong,Zhu Ran,Zhang Jin,Zhuo Xinyi,Hou Yilong.Establishment of Plant Tissue Cultural Technical System in Nettle Cultivar ’Yizhu’[J].Molecular Plant Breeding,2020(7):2331-2337. |
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| Authors: | Li Jiangzhou Zhuang Jie Jin Xiaotong Zhu Ran Zhang Jin Zhuo Xinyi Hou Yilong |
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| Institution: | (College of Life Science and Technology,Dalian University,Dalian,116622) |
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| Abstract: | ’Yizhu’Nettle,as a annuals or perennial herb,has both edible value and medicinal value.Therefore,it is of great significance to study the rapid propagation technology of nettle tissue culture.In this study,the tender tip stem section of the’Yizhu’nettle was explants,the sterilization was carried out by using sodium hypochlorite and ethanol sterilization technology.On the basis of the study on the initial culture,breeding culture and rooting culture conditions,the tissue culture technology system for the rapid propagation of the’Yizhu’nettles was established.The system was that 2%sodium hypochlorite 5 min+75%ethanol 0.5 min is the optimum method of sterilization.MS+0.25 mg/L 6-BA+0.25 mg/L IBA+0.25 mg/L KT+0.25 mg/L trans-zeatin+0.7%agar+3%sucrose was the optimal medium for the first generation.2/3 MS+0.5 mg/L 6-BA+0.3 mg/L IBA+0.25 mg/L trans-zeatin+0.7%agar+3%sucrose was the optimal subculture medium.MS+0.5 mg/L 6-BA+1.0 mg/L NAA+0.7%agar+3%sucrose was the optimal rooting medium. |
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| Keywords: | ’Yizhu’nettle(Urtica dioica) Tender tip stem section Tissue culture |
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