| 糜子农艺性状及SSR分子标记的遗传多样性 |
| |
| 引用本文： | 郭世华,高志军,李涛,陈重,李星聪.糜子农艺性状及SSR分子标记的遗传多样性[J].分子植物育种,2020(6):1974-1982. |
| |
| 作者姓名： | 郭世华 高志军 李涛 陈重 李星聪 |
| |
| 作者单位： | 内蒙古农业大学农学院;内蒙古鄂尔多斯市农牧业科学研究院 |
| |
| 基金项目： | 国家自然科学基金项目(31560394,31860409);内蒙古农业大学科技成果转化专项资金动植物品种选育项目(YZGC2017021)共同资助。 |
| |
| 摘 要： | 为了明确引进糜子种质资源的遗传多样性,以来自中国不同生态区的22份糜子品种为材料,利用SPSS软件和SSR分子标记进行农艺性状相关性、方差分析和聚类分析。结果表明,(1)农艺性状的变异范围为6.54%~45.43%,变异幅度由大到小分别为:穗粒重>单株穗重>单株草重>亩产量>茎粗>主穗长>主茎节数>主茎长>千粒重;主茎长与主穗长、单株穗重、主茎节数、茎粗、单株草重、穗粒重呈极显著正相关,穗粒重与主茎长、主穗长、单株穗重、主茎节数、茎粗、单株草重、千粒重呈极显著正相关,但均与亩产量无显著相关性。‘陇糜11’、‘宁糜13’、‘宁糜14’、‘固糜21’和‘赤糜2号’等5份品种的亩产量大于其余17份品种,且差异达到显著水平;‘陇糜9号’、‘宁糜16’、‘宁糜17’、‘内糜5号’、‘榆糜2号’等5份品种的亩产量相对较低。(2)以阈值2.0将供试22份糜子材料分为5大类群。第一类亩产量最低包括‘宁糜16’‘、宁糜17’‘、榆糜2号’;第五类亩产量最高,包括上述‘陇糜11’等5份品种,其中‘陇糜11’和‘宁糜14’的亩产量分别为3 847.35kg/hm^2和3 786.75 kg/hm^2。(3)SSR分子标记检测到BM552等17对多态性较好的SSR引物,参试品种的相似性系数在0.67~0.99之间,在相似性系数为0.77处,将糜子品种分为6大类群。其中第一类群包括‘陇糜5号’等15份品种,来源于西北春糜子区、呼和浩特和赤峰。第二类群包括‘陇糜11’、‘宁糜13’、‘宁糜9号’。第三、第四、第五、第六类群各有1个品种,依次分别为‘内糜6号’、‘陇糜10号’、‘宁糜16’、‘伊糜5号’。
|
| 关 键 词： | 糜子(Panicum miliaceum L.) 农艺性状 SSR 遗传多样性 |
Genetic Diversity of Agronomic Traits and SSR Markers in Common Millet |
| |
| Guo Shihua,Gao Zhijun,Li Tao,Chen Zhong,Li Xingcong.Genetic Diversity of Agronomic Traits and SSR Markers in Common Millet[J].Molecular Plant Breeding,2020(6):1974-1982. |
| |
| Authors: | Guo Shihua Gao Zhijun Li Tao Chen Zhong Li Xingcong |
| |
| Institution: | (Agricultural College,Inner Mongolia Agricultural University,Hohhot,010019;Inner Mongolia Eerduosi Academy of Agricultural and Animal Sciences,Dalateqi,017200) |
| |
| Abstract: | In orde r to clarify the genetic diversity of common millet germplasm resources,22 common millet cultivars from Chinese different ecological regions were used for correlation,variance and cluster analysis in agronomic traits by using SPSS software and SSR molecular markers.The results were showed that(1)Variation range of agronomic traits was 6.54%~45.43%.The variation ranges of agronomic traits from big to small were:spike grain weight>panicle weight per plant>weight per plant grass>area yield>thick stems>main ear length>number of main stem section>main stem length;Main stem length had very significant positive correlation with main spike length,spike weight per plant,number of main stem section,stem thickness and weight per plant grass.The yield of’Longmei11’,’Ningmei13’,’Ningmei14’,’Gumei21’and’Chimei2’was the highest than that of the other17 cultivars,and had significant difference with the others.The yield of’Longmei9’,’Ningmei16’,’Ningmei17’,’Neimei5’,’Yumei2’was lower than that of the others.(2)22 common millet were divided into five categories by threshold value 2.0.The first class had lower yield than that of other cultivars,including’Ningmei16’,’Ningmei17’and’Yumei2’.The fifth group had the highest yield including’Longmei11’,’Ningmei14’,’Gumei21’,’Chimei2’and’Ningmei13’,of them the yield of’Longmei11’and’Ningmei14’were 3847.35 kg/hm^2 and 3786.75 kg/hm^2,respectively.(3)17 pairs of better polymorphic SSR primers(for example BM552)were detected by SSR molecular markers.Ranges of similarity coefficients were 0.67~0.99 in tested common millet varieties,which were divided into 6 types at similarity coefficient 0.77.First groups included 15 cultivars,such as’Longmei5’and so on,which were from the Northwest Spring Broomcorn Millet Area,Hohhot and Chifeng.The second groups were’Longmei11’,’Ningmei13’,’Ningmei9’.Each class had one cultivars from the third to sixth class,associated with’Neimei6’,’Longmei10’,’Ningmei16’and’Yimei5’,respectively. |
| |
| Keywords: | Common millet(Panicum miliaceum L ) Agronomic traits SSR Genetic diversity |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
