| 甘蔗乙烯信号转导途径关键基因CTR1的克隆与表达分析 |
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| 引用本文： | 王凡伟,肖冬,李杨瑞,何龙飞,王爱勤.甘蔗乙烯信号转导途径关键基因CTR1的克隆与表达分析[J].分子植物育种,2020(3):827-833. |
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| 作者姓名： | 王凡伟 肖冬 李杨瑞 何龙飞 王爱勤 |
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| 作者单位： | 广西大学农学院;广西农业科学院甘蔗研究所 |
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| 基金项目： | 国家自然科学基金(81860670);广西科技项目(GKAD17195100);国家现代农业产业技术体系广西甘蔗创新团队项目(gjnytxgxcxtd-03-01)共同资助。 |
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| 摘 要： | 为了研究CTR1基因与甘蔗糖分积累的关系,本研究利用同源克隆的方法,以甘蔗品种‘桂糖28’(GT28)幼嫩叶片的cDNA为模板,成功克隆得到2103 bp的甘蔗CTR1基因(ScCTR1;GenBank登录号为MK158246)。ScCTR1基因的开放阅读框(ORF)序列长1656 bp,编码551个氨基酸,预测蛋白质的分子质量为62.78 kD。ScCTR1蛋白具有CTR1同源蛋白典型的丝氨酸/苏氨酸蛋白激酶结构域。实时荧光定量PCR结果表明,ScCTR1基因在茎和叶中都有表达,在茎中其表达量呈先增加后降低的趋势,在未成熟叶、成熟叶和老叶中,其基因表达呈降低的趋势。本研究结果对阐明乙烯调控甘蔗茎糖分积累和叶发育的机理提供了重要的参考依据。
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| 关 键 词： | 甘蔗(Saccharum ssp.Hybrids) CTR1 基因克隆 基因表达 |
Cloning and Expression Analysis of the Gene of a Key Factor CTR1 in Sugarcane Ethylene Signal Transduction Pathway |
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| Wang Fanwei,Xiao Dong,Li Yangrui,He Longfei,Wang Aiqin.Cloning and Expression Analysis of the Gene of a Key Factor CTR1 in Sugarcane Ethylene Signal Transduction Pathway[J].Molecular Plant Breeding,2020(3):827-833. |
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| Authors: | Wang Fanwei Xiao Dong Li Yangrui He Longfei Wang Aiqin |
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| Institution: | (State Key Laboratory for Conservation and Uilization of Subtropical Agro-Bioresource,Collge of Agriculture,Guangxi University,Nanning,530005;Guangxi Key Laboratory of Sugarcane Genetic Improvement,Key Laboratory of Sugarcane Biotechnology and Genetic Improvement(Guangxi),Ministry of Agriculture,Sugarcane Rescarch Center,Sugarcane Rescarch Institute,Chinese Academy of Agricultural Sciences,Guangxi Academy of Agriculture Science,Nanning,530007) |
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| Abstract: | In order to study the relationship between CTR1 gene and sugar accumulation in sugarcane.In the present study,we successfully cloned a 2103 bp of sugarcane CTR1 gene(ScCTR1;GenBank accession number is MK158246)from tender leaves of a sugarcane cultivated varieties GT28 by homologous cloning method.The open reading frame sequence of ScCTR1 is 1656 bp in length,encoding 551 amino acids.The molecular mass of deduced sugarcane CTR1 protein is 62.78 kD.Protein domain analysis showed that ScCTR1 had a serine/threonine protein kinase domain which is a typical domain in CTR1 homologous proteins.qRT-PCR results showed that ScCTR1 was expressed in both stem and leaf,and the expression level of ScCTR1 in stem increased first and then decreased,while in immature leaves,maturing leaves and matured leaves,the gene expression showed a decreasing trend.The results from this study provide an important reference to illustrate the mechanism of ethylene regulated sucrose accumulation in stalks and leaf development of sugarcane. |
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| Keywords: | Sugarcane(Saccharum ssp hybrids) CTR1 Gene cloning Gene expression |
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