| 水稻抗坏血酸氧化酶基因家族生物信息学分析 |
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| 引用本文： | 柯笑楠,胡海超,黄霞,贾玉芳.水稻抗坏血酸氧化酶基因家族生物信息学分析[J].分子植物育种,2020(1):11-19. |
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| 作者姓名： | 柯笑楠 胡海超 黄霞 贾玉芳 |
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| 作者单位： | 浙江农林大学农业与食品科学学院 |
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| 基金项目： | 国家自然科学基金项目(31471431);浙江农林大学“青年拔尖人才”资助项目共同资助 |
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| 摘 要： | 抗坏血酸氧化酶(ascorbic acid oxidase,AAO)是多铜氧化酶家族中的一员,在植物生长发育过程中起重要作用。为深入研究水稻AAO基因家族的功能特征及表达模式,采用生物信息学从Phytozome的水稻数据库鉴定出14个AAO成员,并对其染色体定位、蛋白理化性质及二级结构、基因结构、保守基序、系统发生树和表达模式等方面进行预测分析。结果显示,14个OsAAO不均匀地分布在8条染色体上,多为碱性蛋白;蛋白二级结构以无规则卷曲为主要组成部分;基因结构和保守基序分析表明,OsAAO内含子数目变化差异较大,但氨基酸序列具有较强的保守性;系统发生树可分为3个亚家族,OsAAOs与玉米、高粱簇在一起,亲缘关系较近;另外表达模式分析发现,OsAAO在不同的组织部位表达水平具有差异,这表明其行使功能不同。这些结果为今后研究该基因家族的生物学功能和培育耐旱品种提供理论依据。
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| 关 键 词： | 水稻(Oryza sativa) AAO 基因家族 生物信息学分析 |
Bioinformatics Analysis of Ascorbic Acid Oxidase Gene Family in Oryza sativa L. |
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| Institution: | (College of Agriculture and Food Science,Zhejiang A&F University,Lin'an,311300) |
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| Abstract: | Ascorbic acid oxidase(AAO)is a member of multicopper oxidase family and plays an important role in the growth and development of plant.To further investigated the functional characteristics and expression profiles of AAO gene family in rice,14 members of AAO gene family were identified by bioinformatics based on rice database in Phytozome.And their chromosome localization,physicochemical properties,secondary structure of protein,gene structure,conserved motif,phylogenetic tree and expression profiles were also predicted and analyzed.The results showed that 14 OsAAO genes were distributed unequally in 8 chromosomes and most of them were alkaline protein.The secondary structure of OsAAO genes was mainly random coil.Gene structure and conserved motif analysis indicated that the intron number of OsAAO genes was different,but the amino acid sequences were highly conserved.Phylogenetic tree can be divided into three subfamilies,and OsAAOs were closely related to Zea mays and Sorghum bicolor.Additionally,expression profiles analysis founded that AAO genes showed diverse expression levels in different parts of rice,suggesting a significant difference in their function.These results will provide theoretical support for future functional researches of AAO gene family as well as cultivating drought-tolerant varieties. |
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| Keywords: | Rice(Oryza sativa) AAO Gene family Bioinformatics analysis |
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