Inheritance and field performance of transgenic Korean Bt rice lines resistant to rice yellow stem borer |
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| Authors: | Songjin Kim Choljun Kim Wonnam Li Tokyong Kim Yongsu Li Mohsin Abbas Zaidi Illimar Altosaar |
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| Institution: | (1) Biotechnology Laboratory, Research Institute of Agrobiology, Academy of Agricultural Sciences, Pyongyang, Democratic People’s Republic of Korea;(2) Biology Faculty, Pyongyang University of Agriculture, Pyongyang, Democratic People’s Republic of Korea;(3) Agricultural Biotechnology Laboratory, Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, ON, Canada, K1H 8M5 |
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| Abstract: | Transgenic Korean rice plants containing the cry1Ab gene were developed for resistance against yellow stem borer (Scirpophaga incertulas, YSB). More than 100 independent transgenic lines from three Korean varieties (P-I, P-II and P-III) were generated. The amount
of Cry1Ab in transgenic T0 plants was as high as 2.88% of total soluble proteins. These levels were sufficient to cause 100% mortality of YSB larvae.
The majority of T1 transgenic lines originated from the varieties P-I and P-II followed a Mendelian fashion of segregation. Deviation from the
expected segregation ratio was observed in a small number of the transgenic lines of P-I and P-II origins. However, this deviation
was primarily observed in the P-III originated lines. Segregation analysis of the T1 generation indicated that 1–3 copies of the cry1Ab gene were integrated into the genome of the majority of the transgenic lines originating from varieties P-I and P-II. Stunted
and semi-fertile mutants were observed in some transgenic lines. These aberrations were either independent or closely linked
to the introduced cry1Ab gene loci in different transgenic lines. Reduction in GUS expression levels and loss of toxicity against YSB larvae were
found in some transgenic lines. The transgenic T3 and T4 lines causing 100% mortality of third instar YSB larvae in the lab were completely protected in the field. Analysis of important
yield components on nine selected transgenic lines indicated that stem length, panicle length, grain number per panicle, and
seed setting rates were reduced in transgenic plants compared to those in non-transgenic parental rice lines. Number of panicles
per cluster, however, was significantly higher in transgenic plants. The numerical value of the average yield was in general
greater in the controls than in all the transgenic lines, indicating some ‘yield drag’. Since some selected lines were highly
resistant to the YSB with good yielding potential, they offer effective potential for use in insect resistance management
programs. |
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| Keywords: | Rice transformation cry1Ab Transgenic plants Mortality Yield components Insect pests YSB Stem borer |
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