| 卷丹百合RAPD-PCR反应程序的优化研究 |
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| 引用本文： | 韩凌,雷家军.卷丹百合RAPD-PCR反应程序的优化研究[J].河北农业科学,2009,13(2):52-54. |
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| 作者姓名： | 韩凌 雷家军 |
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| 作者单位： | 1. 沈阳职业技术学院,辽宁,沈阳,110045
2. 沈阳农业大学园艺学院,辽宁,沈阳,110161 |
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| 摘 要： | 采用CTAB法提取卷丹百合的基因组DNA。采用正交设计和单因素试验相结合的方法，对卷丹百合RAPD—PCR反应程序中各影响因素进行了优化。结果表明：卷丹百合最佳的RAPD—PCR扩增程序为94℃预变性4min；94℃变性30s、39℃退火40s、72℃延伸1min，45个循环；72℃延伸10min。
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| 关 键 词： | 卷丹百合 RAPD 正交设计 单因素试验 程序优化 |
Optimization of RAPD-PCR Procedure in Lilium lancifolium Thunb. |
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| HAN Ling,LEI Jia-jun.Optimization of RAPD-PCR Procedure in Lilium lancifolium Thunb.[J].Journal of Hebei Agricultural Sciences,2009,13(2):52-54. |
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| Authors: | HAN Ling LEI Jia-jun |
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| Institution: | 1.Shenyang Polytechnic College;Shenyang110045;China;2.College of Horticulture;Shenyang Agricultural University;Shenyang110161;China |
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| Abstract: | Genomic DNA of Lilium lancifolium Thunb. was extracted with the method of CTAB. Some influencing factors in the RAPD reaction procedure were studied with orthogonal design and single factor experiment. The results showed that the optimum RAPD-PCR procedure was one cycle of denaturing at 94 ℃ for 4 min, 45 cycles of denaturing at 94 ℃ for 30 s, annealing at 39 ℃ for 40 s, extending at 72 ℃ for 1 min, one cycle of final extension extending at 72 ℃ for 10 min. |
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| Keywords: | RAPD |
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