| 3种葫芦科作物种质资源中黄瓜绿斑驳花叶病毒的检测 |
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| 引用本文： | 张永江,马洁,李桂芬,李明福.3种葫芦科作物种质资源中黄瓜绿斑驳花叶病毒的检测[J].种子,2008,27(10). |
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| 作者姓名： | 张永江 马洁 李桂芬 李明福 |
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| 作者单位： | 中国检验检疫科学研究院动植物检疫研究所,北京,100029 |
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| 基金项目： | 国家质量监督检验检疫总局资助项目 |
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| 摘 要： | 为确定黄瓜绿斑驳花叶病毒在葫芦、西瓜、甜瓜3种葫芦科(Cucurbitaceae)作物种质中的携带部位、最低检测量及不同检测技术的检测效果,对3种作物的不同种质资源以及同种种质资源的不同处理进行了DAS-ELISA和RT-PCR检测,结果显示受侵染葫芦种子的种皮、种仁可带毒;受侵染西瓜的瓜叶、瓜皮、瓜瓤、种皮、种仁、瓜蔓均可带毒;受侵染甜瓜的叶片可带毒。RT-PCR检测灵敏度高于ELISA。
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| 关 键 词： | 葫芦科 种质资源 黄瓜绿斑驳花叶病毒 检测 |
Detection Cucumber Green Mottle Mosaic Virus in Three Cucurbitaceae Crops Germplasm Sources |
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| ZHANG Yong-jiang,MA Jie,LI Gui-fen,LI Ming-fu.Detection Cucumber Green Mottle Mosaic Virus in Three Cucurbitaceae Crops Germplasm Sources[J].Seed,2008,27(10). |
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| Authors: | ZHANG Yong-jiang MA Jie LI Gui-fen LI Ming-fu |
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| Abstract: | Detecting three Cucurbitaceae crops different germplasm sources and different treatments of same germplasm sources by DAS-ELISA and RT-PCR,for making sure the taking position of Cucumber green mottle mosaic virus(CGMMV),the minimum detection quantity and detection effects of different technique.The results displayed that seed coat and seed kernel of cucurbits,leaf,husk,flesh,seed coat,seed kernel,and vine of watermelon,leaf of melon were infected by CGMMV.The RT-PCR was more sensitive than DAS-ELISA in detection of cucurbits seed. |
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| Keywords: | Cucurbitaceae germplasm sources Cucumber green mottle mosaic virus detection |
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