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| CAC3基因转运肽序列和accD基因融合植物表达载体的构建 | |
| 引用本文： | 张煜星,崔燕,武寒雪,祝建波,周鹏.CAC3基因转运肽序列和accD基因融合植物表达载体的构建[J].华北农学报,2008,23(5). |
| 作者姓名： | 张煜星 崔燕 武寒雪 祝建波 周鹏 |
| 作者单位： | 1. 中国热带农业科学院,热带生物技术研究所国家重点实验室,海南,海口,571101;石河子大学,生命科学学院,农业生物技术重点实验室,新疆,石河子,832003 2. 石河子大学,生命科学学院,农业生物技术重点实验室,新疆,石河子,832003 3. 中国热带农业科学院,热带生物技术研究所国家重点实验室,海南,海口,571101 |
| 基金项目： | 国家"863"子项目，兵团博士基金 |
| 摘 要： | 采用PCR方法分别从陆地棉和拟南芥基因组中扩增出Accase羧基转移酶β-CT亚基编码基因accD,Accase羧基转移酶α-CT亚基编码基因CAC3的定位于叶绿体的转运肽序列.分析结果与NCBI公布的氨基酸同源性分别为100%,99%.将CAC3基因转运肽序列与accD基因进行体外重组,构建了融合植物表达载体pBI-CAC3tp-accD,为下一步作物的遗传转化打下了基础. |
| 关 键 词： | 陆地棉 拟南芥 accD基因 CAC3基因 转运肽 |
Construction of Plant Expression Vector on Fusion Transit Peptide of CAC3 Gene and accD Gene |
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| ZHANG Yu-xing,CUI Yan,WU Han-xue,ZHU Jian-bo,ZHOU Peng.Construction of Plant Expression Vector on Fusion Transit Peptide of CAC3 Gene and accD Gene[J].Acta Agriculturae Boreali-Sinica,2008,23(5). | |
| Authors: | ZHANG Yu-xing CUI Yan WU Han-xue ZHU Jian-bo ZHOU Peng |
| Institution: | ZHANG Yu-xing1,2,CUI Yan2,WU Han-xue2,ZHU Jian-bo2,ZHOU Peng1 (1.Institute of Tropical Bioscience , Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China,2.Laboratory of Agricultural Biotechnology,College of Life Science,Shihezi University,Shihezi 832003,China) |
| Abstract: | The acetyl-CoA carboxylase(ACCase) is the rate-limiting enzyme of fatty acids synthesis.The accD gene and transit peptide of CAC3 gene was amplified from Gossyiuum hirsutum genome and Arabidopsis thaliana genome,DNA sequence analysis indicated that the cloned DNA fragmes have the highest homology compared with the NCBI published date,showing 100% and 99%.Plant expression vector of fusion transit peptide of CAC3 gene and accD gene was constructed.The vector of pBI-CAC3tp-accD will be usefull for further natu... |
| Keywords: | Gossypium hirsutum Arabidopsis thaliana accD gene CAC3 gene Transit peptide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |