| 棉花微卫星标记的PAGE/银染快速检测 |
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| 引用本文： | 张军,武耀廷,郭旺珍,张天真.棉花微卫星标记的PAGE/银染快速检测[J].棉花学报,2000,12(5):267-269. |
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| 作者姓名： | 张军 武耀廷 郭旺珍 张天真 |
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| 作者单位： | 农业部农作物种质资源与育种重点开放实验室,南京农业大学农学系,南京,210095 |
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| 基金项目： | 国家自然科学基金(39830240，3980089)，国家转基因植物研究专项(J99-A-023)资助项目 |
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| 摘 要： | 利用一种简便、快速的PAGE／银染方法检测了异源四倍体棉花栽培种陆地棉与海岛棉之间的微卫星多态性，并检测到了微卫星在陆地棉品种间的多态及微卫星标记位点在陆地棉品种间的复等位性。
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| 关 键 词： | 棉花 微卫星 PAGE 银染 |
| 文章编号： | 1002-7807(2000)05-0267-03 |
| 修稿时间： | 2000-06-24 |
Fast Screening of Microsatellite Markers in Cotton with PAGE/silver Staining |
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| ZHANG Jun,WU Yao-ting,GUO Wang-zhen,ZHANG Tian-zhen.Fast Screening of Microsatellite Markers in Cotton with PAGE/silver Staining[J].Cotton Science,2000,12(5):267-269. |
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| Authors: | ZHANG Jun WU Yao-ting GUO Wang-zhen ZHANG Tian-zhen |
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| Institution: | ZHANG Jun
,WU Yao-ting
,GUO Wang-zhen
,ZHANG Tian-zhen
;(Agronomy Department,Nanjing Agricultural University; Key Laboratory of Crop Germplasm and Breeding, Ministry of Agriculture,Nanjing 210095, China) |
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| Abstract: | Polymorphism of microsatellite markers between cultivars of allotetraploid cotton species (Gossypium hirsutum L. ) and the seaisland cotton (G. babardense L. ) was screened using a rapid and simple method of PAGE/silver staining. The polymorphic and multiallelic character of microsatellite markers among upland cotton cultivars were identified as well. |
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| Keywords: | cotton micosatellite PAGE silver staining |
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