| 小立碗藓细胞色素P450基因PpCYP51G1的分离及转化载体构建 |
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| 引用本文： | 丁谦,叶帅,李景娟,朱正歌,高建伟.小立碗藓细胞色素P450基因PpCYP51G1的分离及转化载体构建[J].山东农业科学,2013(10):1-6,11. |
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| 作者姓名： | 丁谦 叶帅 李景娟 朱正歌 高建伟 |
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| 作者单位： | 山东省农业科学院蔬菜研究所/山东省设施蔬菜生物学重点实验室/国家蔬菜改良中心山东分中心;甘肃农业大学;河北师范大学生命科学学院; |
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| 基金项目： | 国家自然科学基金(30671177);山东省优秀中青年科学家科研奖励基金(2006BS06001);山东省农业科学院博士基金(2006-2009)资助 |
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| 摘 要： | 从小立碗藓（Physcomitrella patens）中克隆了细胞色素P450 CYP51基因PpCYP51G1的全长编码序列，编码区长1509bp，预测编码488个氨基酸，蛋白二级结构含有自由卷曲、伸展片段和α-螺旋，N端含有一个跨膜区域。构建了PpCYP51G1的功能缺失载体，并利用PEG介导的原生质体转化技术获得该基因的功能缺失转化体；同时，构建了印CYP51G1-GFP融合蛋白载体，亚细胞定位结果表明，PpCYP51G1定位在内质网中。
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| 关 键 词： | 小立碗藓 细胞色素P450 CYP51 克隆 转化载体构建 |
Isolation of Cytochrome P450 Gene PpCYP51G1 from Physcomitrella patens and Construction of Its Transformation Vectors |
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| Ding Qian,Ye Shuai,Li Jingjuan,Zhu Zhengge,Gao Jianwei.Isolation of Cytochrome P450 Gene PpCYP51G1 from Physcomitrella patens and Construction of Its Transformation Vectors[J].Shandong Agricultural Sciences,2013(10):1-6,11. |
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| Authors: | Ding Qian Ye Shuai Li Jingjuan Zhu Zhengge Gao Jianwei |
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| Institution: | 1. Institute of Vegetables, Shandong Academy of Agricultural Sciences/Shandong Key Laboratory of Greenhouse Vegetable Biology/Shandong Branch of National Vegetable Center, Jirmn 250100, China; 2. Gansu Agricultural University, Lanzhou 730070, China ; 3. College of Life Science, Hebei Normal University, Shijiazhuang 050016 , China) |
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| Abstract: | The full length cDNA sequence of cytochrome P450CYP51 gene named as PpCYP51G1 was cloned from Physcomitrella patens. Its code region was 1 509 bp, and it encoded 488 amino acids. The PpCYP51 G1 protein contained one transmembrane region at the N end, and its secondary structure contained random coils, extended strands and alpha helixes. The knockout vector for PpCYP51G1 was constructed and the loss - of - function transformants were obtained. The expression vector for PpCYP51G1 - GFP fusion was also constructed to investigate the subcellular localization of PpCYP51G1, and the result showed that PpCYP51G1 expressed in endoplasmic reticulum. |
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| Keywords: | Physcomitrella patens Cytochrome P450 CYP51 Clone Construction of transformation vector |
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