| Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR |
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| 作者姓名： | YANG Guohui ZHANG Zhongkai CUI Chongshi |
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| 作者单位： | 1. Heilongjiang Academy of Agricultural Science，Harbin Heilongjiang 150086，PRC; 2. College of Horticulture，Northeast Agricultural University，Harbin Heilongjiang 150030，PRC; 3. Agricultural Biotechnological Key Laboratory of Yunnan Province，Kunming Yunnan 650223，PRC |
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| 基金项目： | The 0pen Fundation Item of Agricultural Biotechnological Key Laboratory of Yunnan Province （2001B-3） |
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| 摘 要： | Pumpkin is a kind of crop which is often infected by viruses, in the past biology, serum and nucleic acid hybridization methods were mainly used on the study of pumpkin virus disease in China1-2]. A lot of new PCR technologies have developed on base of P…
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| 关 键 词： | 南瓜 病毒性疾病 病原检测 RT-PCR IC-PCR |
| 文章编号： | 1006-8104（2006）-02-0129-04 |
| 收稿时间： | 2006-04-26 |
Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR |
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| YANG Guohui ZHANG Zhongkai CUI Chongshi.Detection and Comparison of Pathogen of Virus Disease in Pumpkin by RT-PCR and IC-PCR[J].Journal of Northeast Agricultural University,2006,13(2):129-132. |
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| Authors: | YANG Guohui ZHANG Zhongkai CUI Chongshi |
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| Abstract: | Two kinds of methods RT-PCR and IC-PCR were used to detect pathogen of virus disease of pumpkin and the sensitivity of the two methods was compared. The results showed that PRSV-W and CMV were detected in diseased samples gathered in Yunnan Province, while WMV and CMV were detected in diseased samples gathered in Heilongjiang Province. The sensitivity of RT-PCR is higher than that of IC-PCR, but the effect of IC-PCR in the specialization of bonding reaction and requisition for experiment material is better than that of RT-PCR. |
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| Keywords: | pumpkin virus disease RT-PCR IC-PCR |
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