| 显微技术在植物Al毒研究中的应用 |
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| 引用本文： | 孙清斌,赵学强,沈仁芳.显微技术在植物Al毒研究中的应用[J].土壤学报,2009,46(6):1026-1032. |
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| 作者姓名： | 孙清斌 赵学强 沈仁芳 |
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| 作者单位： | 1. 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008;中国科学院研究生院,北京,100049
2. 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008 |
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| 基金项目： | 国家自然科学基金项目，中国科学院知识创新工程重要方向项目 |
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| 摘 要： | 分析比较了苏木精染色、荧光染色和扫描电镜3种显微观察技术在研究胡枝子A l胁迫下根尖形态变化中的优缺点。结果表明,苏木精染色法可以区分两种胡枝子品种及不同A l处理间的差异;荧光染色法可以显著区分对照和A l处理间的差别,而且在观察根表黏胶物质和A l在质外体分布时极为方便;扫描电镜可以区分A l处理同对照间的差别,但不能通过能谱分析区分品种间的耐A l性差异。
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| 关 键 词： | Al毒 显微技术 胡枝子 |
Application of three microscopic techniques to research on Al toxicity in plants |
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| Sun Qingbin,Zhao Xueqiang and Shen Renfang.Application of three microscopic techniques to research on Al toxicity in plants[J].Acta Pedologica Sinica,2009,46(6):1026-1032. |
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| Authors: | Sun Qingbin Zhao Xueqiang and Shen Renfang |
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| Institution: | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences; Graduate School of the Chinese Academy of Sciences;State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences;State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences |
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| Abstract: | Aluminum (Al) toxicity often exists in plants growing on acid soils, limiting crop production therein. Microscopic techniques are commonly used in the research on Al toxicity in plants. A comparison study was carried out of the advantages and disadvantages of three microscopic techniques, hematoxylin staining method, fluorescent staining method and electronic microscopic scanning method, using growing under Al stress. Results show that the hematoxylin staining method could distinguish difference in morphologic variation of root tips between two different cultivars of lespedezas and between different Al treatments. The fluorescent staining method could detect differences between plants in control and in Al treatments, and make it convenient to observe mucilage on root surface and Al distribution in apoplast. The electronic microscopic scanning method could make out differences between the plants in control and in Al treatments, but could not find out difference in Al tolerance between cultivars of lespedezas through energy spectrum analysis. |
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| Keywords: | Al toxicity Micrograph Lespedeza |
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