| 诱变与组织培养相结合诱发水稻耐盐突变的初步研究 |
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| 引用本文： | 田少华,梁竹青,高明尉,项友斌.诱变与组织培养相结合诱发水稻耐盐突变的初步研究[J].核农学报,1988,2(2):65-72. |
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| 作者姓名： | 田少华 梁竹青 高明尉 项友斌 |
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| 作者单位： | 浙江农业大学核农所,浙江农业大学核农所,浙江农业大学核农所,浙江农业大学核农所 杭州 ,杭州 ,杭州 ,杭州 |
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| 摘 要： | 以4个水稻品种的成熟种子作为外植体,用γ射线、叠氮化钠(SA)施行单项或复合诱变处理后进行组织培养,出愈后的部分愈伤组织再用γ射线辐照处理,并在含NaCl的筛选培养基上进行耐盐突变的筛选。结果表明,15kRγ射线对出愈率和生长率均有严重的抑制作用,而1mMSA单独处理对出愈率和生长率则有促进作用,所有诱变处理都降低了植株分化频率,愈伤组织的生长速率和植株再生率虽随筛选培养基中NaCl含量的提高而下降,但1％NaCl作为耐盐突变体的筛选压较为适宜。诱变处理的种子愈伤组织再以适当剂量的γ射线辐照,有提高诱发耐盐突变频率的作用。适宜的照射量为500—750R。
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| 关 键 词： | 水稻 诱变剂 愈伤组织 耐盐突变 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
PRELIMINARY STUDIES ON THE APPLICATION OF MUTAGENS AND IN-VITRO TECHNIQUES TO RICE SEEDS FOR INDUCTING SALT TOLERANT MUTANTS |
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| Abstract: | The mature seeds of four rice varieties were used as explants in this study. Dehulled rice seeds were treated with gamma-rays irradiation and sodium azide either in combinated way or alone. Calli derived from "IR28" and "Zhenshen 97B" were also irradiated with γ-rays.The results show that the callus induction rate and callus growth rate were seriously inhibited when the seeds had been exposured to 15kR Y-rays, 1 mM sodium azide ( SA ) had some stimulative effects on these two parameters. It also indicated that the plantlet regeneration frequency decreased while all the mutagenic treatments employed. The callus growth rate and plantlet regeneration frequency decreased with the increasing of NaCl content in salt enriched selective medium.The concentration of 1 % NaCl(W/V) in medium is considered as optimal stress level to identify and isolate salt-tolerant callus lines.These lines maintained their capacity of plant regeneration.The optimal radiation dosage for treating callus derived from rice seeds ranges 500-750R. |
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| Keywords: | rice(oryza sativa L) mutagen callus mutation of salt-tolerance |
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