| 鸭TYR基因序列的克隆与分析 |
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| 引用本文： | 朱志明,郑嫩珠,缪中纬,郑丽祯,李盛霖,陈晖.鸭TYR基因序列的克隆与分析[J].福建农业学报,2008,23(3). |
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| 作者姓名： | 朱志明 郑嫩珠 缪中纬 郑丽祯 李盛霖 陈晖 |
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| 作者单位： | 福建省农业科学院畜牧与兽医研究所,福建,福州,350013 |
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| 基金项目： | 福建省科技重点项目(2005N023); 福建省科技重大专项专题(2006NZ0003-3) |
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| 摘 要： | 根据鸡和鹌鹑的酪氨酸酶基因（TYR）保守序列设计1对引物，以半番鸭基因组为模板进行PCR扩增，将扩增出的序列进行克隆和测序，结果得到长为630bp的序列。序列分析表明该基因片段包含外显子1部分序列，与鸡、鹌鹑和孔雀的TYR基因分别有93．5％、94．3％和93．4％的同源性，充分显示了TYR基因在进化上的保守性；对扩增序列进一步研究发现第133处存在G／A突变。该基因部分序列的克隆，为进一步开展研究TYR基因多态性与鸭羽色的相关性研究奠定基础。
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| 关 键 词： | 鸭 TYR基因 克隆 序列分析 |
Cloning and sequence analysis of duck TYR Gene |
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| ZHU Zhi-ming,ZHENG Nen-zhu,MIAO Zhong-wei,ZHENG Li-zhen,LI Sheng-lin,CHEN Hui.Cloning and sequence analysis of duck TYR Gene[J].Fujian Journal of Agricultural Sciences,2008,23(3). |
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| Authors: | ZHU Zhi-ming ZHENG Nen-zhu MIAO Zhong-wei ZHENG Li-zhen LI Sheng-lin CHEN Hui |
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| Abstract: | According to the TYR gene conserved sequences of chicken and quails,a pair of primers was designed to amplify the sequence of TYR gene from mule duck genomic DNA.The fragment was cloned and sequenced to show 630 bp in length.The fragment included partial exon1.The homologous comparison among duck,chicken,quail and peacock,indicated that the duck's TYR gene was 93.5%,94.3% and 93.4%homologous with chicken,quail and peacock,respectively,suggesting the conservative in phylogeny of the TYR gene.A G/A mutation a... |
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| Keywords: | duck TYR gene clone sequence analysis |
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