| 异育银鲫感染鲤疱疹病毒Ⅱ型(CyHV-2)后血液生理生化指标变化的研究 |
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| 引用本文： | 刘文枝,曾令兵,张 辉,杨 星,徐 进,江 南,周 勇,马 杰.异育银鲫感染鲤疱疹病毒Ⅱ型(CyHV-2)后血液生理生化指标变化的研究[J].上海海洋大学学报,2013,22(6):928-935. |
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| 作者姓名： | 刘文枝 曾令兵 张 辉 杨 星 徐 进 江 南 周 勇 马 杰 |
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| 作者单位： | 上海海洋大学 水产与生命学院,上海海洋大学 水产与生命学院,中国水产科学研究院 长江水产研究所,南京农业大学渔业学院,中国水产科学研究院 长江水产研究所,中国水产科学研究院 长江水产研究所,中国水产科学研究院 长江水产研究所,中国水产科学研究院 长江水产研究所 |
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| 基金项目： | 国家大宗淡水鱼类产业技术体系建设专项资金项目(CARS-46-11) |
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| 摘 要： | 鲤疱疹病毒Ⅱ型（Cyprinid herpesvirusⅡ, CyHV-2）感染异育银鲫（Carassius auratus gibelio）是近年新出现的一种严重病毒性疾病，已经造成重大经济损失，目前缺乏有关该病发病机理的相关研究。以CyHV 2感染异育银鲫的患病鱼内脏组织超微过滤匀浆液，腹腔注射感染健康异育银鲫，分别对感染组与对照组鲫尾静脉采血，进行白细胞分类计数、吞噬细胞活性检测以及血清生化指标测定与分析。结果表明，与对照组相比较，感染组鲫血细胞总数变化不明显，但单核细胞和淋巴细胞百分比显著上升，其中淋巴细胞上升呈极显著差异（P＜0.01），单核细胞上升呈显著差异（0.01＜P＜0.05）；嗜中性粒细胞及血栓细胞显著下降，均呈显著性差异（0.01＜P＜0.05）。单核细胞的吞噬指数与吞噬百分比极显著高于对照组（P＜0.01），红细胞的吞噬指数与吞噬百分比显著高于对照组（0.01＜P＜0.05）。感染组鲫血清总蛋白为（34.32±2.52）mmol/L，对照组为（28.00±2.17）mmol/L，呈显著差异（0.01＜P＜0.05）；血清中谷丙转氨酶为（21.50±1.58）U/L，谷草转氨酶为（407.50±4.52）U/L，碱性磷酸酶为（51.00±5.27）U/L，极显著高于相应的对照组（12.67±1.32）U/L，（346.50±3.07）U/L，（17.50±4.88）U/L（P＜0.01）；血清中甘油三酯、总胆固醇及血糖则均低于对照组，但差异不显著（P＞0.05）。
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| 关 键 词： | 异育银鲫 鲤疱疹病毒Ⅱ型 白细胞分类比 吞噬活性 血清生化指标 |
| 收稿时间： | 2013/8/18 0:00:00 |
| 修稿时间： | 2013/9/23 0:00:00 |
Studies on the change of blood biochemical indices in gibel carp,Carassius auratus gibelio,infected with Cyprinid herpesvirusⅡ |
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| LIU Wen-zhi,ZENG Ling-bing,ZHANG Hui,YANG Xing,XU Jin,JIANG Nan,ZHOU Yong and MA Jie.Studies on the change of blood biochemical indices in gibel carp,Carassius auratus gibelio,infected with Cyprinid herpesvirusⅡ[J].Journal of Shanghai Ocean University,2013,22(6):928-935. |
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| Authors: | LIU Wen-zhi ZENG Ling-bing ZHANG Hui YANG Xing XU Jin JIANG Nan ZHOU Yong and MA Jie |
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| Institution: | College of Fisheries and Life Science,Shanghai Ocean University,College of Fisheries and Life Science,Shanghai Ocean University,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Science,Fishery College,Nanjing Agricultural University,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Science,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Science,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Science,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Science |
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| Abstract: | The infection of cyprinid herpesvirus Ⅱ （CyHV 2） in cultured gibel carp （Carassius auratus gibelio） is a newly emerged severe disease and has caused huge economic losses. Currently, little information about the pathogenesis of disease is available. In this study, the healthy gibel carp was peritoneally injected with the ultra filtrated homogenate from the inner organs of diseased gibel carp infected by CyHV-2. 3 days later, the periphery blood from the infected gibel carp and the control group were collected for the investigation of blood biochemical indices, including haematocyte number, differential leucocyte count （DLC）, phagocytic activity of phagocytes and serum biochemical indices, etc. The results showed, compared with the control group, the total cell number in periphery blood of the artificially infected fish had no apparent change, but the percentages of monocyte and lymphocyte were increased significantly, in which the lymphocyte percentage indicated highly significant difference（P〈0.01）and the monocyte percentage was of significant difference（0.01〈P〈0.05）; The percentages of neutrophilic granulocyt and thrombocyte in the infected group were declined at significant level（0.01〈P〈0.05）. Meanwhile, the phagocytic percentage（PP） and phagocytic index （PI） of monocyte in the infected group was significantly higher than that of the control group（P〈0.01）, and the PP and PI of erythrocyte showed significant difference（0.01〈P〈0.05）.The total protein in the serum of infected fish was （34.32±2.52）mmol/L, which was significantly higher than that in the control group（28.00±2.17）mmol/L（0.01〈P〈0.05）; Highly significant increases of alanine aminotransferase（ALT）, aspartate aminotransferase（AST） and alkaline phosphatase were found in the infected fish compared to the healthy fish（P〈0.01）.The concentrations of ALT,AST and ALP were （21.50±1.58）U/L,（407.50±4.52）U/L and （51.00±5.27）U/L in the challenged fish, respectively, while in the control group, they were （12.67±1.32）U/L,（346.50±3.07）U/L and （17.50±4.88）U/L. In contrast, the concentrations of triglyceride, total cholesterol and blood glucose in the infected group were declined, but showing no significant difference（P〉0.05）. |
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| Keywords: | Carassius auratus gibelio Cyprinid herpesvirusⅡ percentage in leucocytes phagocytic activity of phagocytes serum biochemical indexes |
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