| 梨基因组DNA提取方法比较研究 |
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| 引用本文： | 刘遵春,包东娥,廖明安.梨基因组DNA提取方法比较研究[J].华北农学报,2006,21(4):48-50. |
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| 作者姓名： | 刘遵春 包东娥 廖明安 |
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| 作者单位： | 河南科技学院,园林学院,河南,新乡,453003;河南科技学院,数学系,河南,新乡,453003;四川农业大学,林学园艺学院,四川,雅安,625014 |
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| 摘 要： | 通过添加Vc对传统的CTAB法进行改进,结果表明:在磨样时,1 g鲜样中添加0.10 g的Vc提取的DNA质量最高;最佳提取材料是嫩芽,其次是新梢和成熟叶片。
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| 关 键 词： | 金花梨 基因组DNA 提取 |
| 文章编号： | 1000-7091（2006）04-0048-03 |
| 收稿时间： | 2005-11-17 |
| 修稿时间： | 2005-11-17 |
Comparison of Pear Genomic DNA Extraction Methods |
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| LIU Zun-chun,BAO Dong-e,LIAO Min-gan.Comparison of Pear Genomic DNA Extraction Methods[J].Acta Agriculturae Boreali-Sinica,2006,21(4):48-50. |
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| Authors: | LIU Zun-chun BAO Dong-e LIAO Min-gan |
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| Abstract: | Traditional CTAB method was improved by adding Vc in this experiment.The results showed that the best quality of DNA extracted from pear genome came from the method of adding 0.1 g/g of Vc when the sample was triturated.The DNA was optimal for RAPD analysis and other molecular biology researches.The results of the experiment also indicated that tender bud was the best material,which was followed by the shoot and mature leaf. |
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| Keywords: | Jinhua pear Genomic DNA Extraction method |
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