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Genotyping of Giardia intestinalis from domestic and wild animals in Japan using glutamete dehydrogenase gene sequencing
Authors:Itagaki Tadashi  Kinoshita Shizuka  Aoki Mikiko  Itoh Naoyuki  Saeki Hideharu  Sato Naoto  Uetsuki Junya  Izumiyama Shinji  Yagita Kenji  Endo Takuro
Institution:Laboratory of Veterinary Parasitology, Faculty of Agriculture, Iwate University, 3-18-8 Ueda, Morioka 020-0105, Japan. itagaki@iwate-u.ac.jp
Abstract:To determine the genotypes of Giardia intestinalis from domestic and wild animals in Japan, Giardia isolates obtained from feces of 24 dogs kept in households and breeding kennels, three companion cats, five dairy calves and three wild monkeys, Macaca fuscata, were genotyped using the 177 bp sequence of the glutamete dehydrogenase gene (gdh). The genotypes were assemblages A, C, D or A/D for dog isolates, Assemblage F for cat isolates, assemblages A or E for calf isolates and assemblage B for monkey isolates. This is the first report on the genotypes of Giardia isolates from cats, calves and wild monkeys in Japan.
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