| 抗小鼠H-Y抗原IgY抗体的纯化 |
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| 引用本文： | 刘本成,药晨江,雷庆,李欣,周颖,徐汪节,李巧丽,王朝霞,乔中东.抗小鼠H-Y抗原IgY抗体的纯化[J].上海交通大学学报(农业科学版),2008,26(4):272-276. |
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| 作者姓名： | 刘本成 药晨江 雷庆 李欣 周颖 徐汪节 李巧丽 王朝霞 乔中东 |
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| 作者单位： | 上海交通大学,生命科学技术学院,上海,200240 |
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| 基金项目： | 上海市科技兴农科技攻关项目 |
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| 摘 要： | 用Ni—NTA柱纯化SMCY重组蛋白，将该蛋白作为配基偶联至CNBr活化的Sepharose 4B制成亲和层析柱，分离纯化抗H—Y抗原的特异性IgY抗体。结果表明：Ni—NTA柱纯化后，SMCY重组蛋白纯度可达到80％左右；亲和层析柱纯化可以使IgY抗体的纯度达到97％，经免疫组化检测，该抗体能够结合与雄性小鼠肝脏细胞表面，而不与雌性小鼠肝脏细胞发生结合。证实了经过纯化得到的高纯度IgY抗体具有很好的抗H—Y抗原特异性。通过该方法可纯化分离得到高纯度抗H—Y抗原IgY抗体。
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| 关 键 词： | H-Y Ni—NTA 亲和层析 IgY |
Purification of Anti-Mouse H-Y Antibody,IgY |
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| LIU Ben-cheng YAO Chen-jiang LEI Qing LI Xin ZHOU Ying XU Qang-jie LI Qiao-li WANG Chao-xia QIAO Zhong-dong.Purification of Anti-Mouse H-Y Antibody,IgY[J].Journal of Shanghai Jiaotong University (Agricultural Science),2008,26(4):272-276. |
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| Authors: | LIU Ben-cheng YAO Chen-jiang LEI Qing LI Xin ZHOU Ying XU Qang-jie LI Qiao-li WANG Chao-xia QIAO Zhong-dong |
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| Institution: | LIU Ben-cheng YAO Chen-jiang LEI Qing LI Xin ZHOU Ying XU Qang-jie LI Qiao-li WANG Chao-xia QIAO Zhong-dong (School of Life Science , Biotectmology,Shanghai Jiaotong University,Shanghai 200240,China) |
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| Abstract: | Purpose Get anti-H-Y antibody of high level of purity,IgY.Method:Purified SMCY reeomhined protein with Ni-NTA chromatography with its 6his-tag at the N-terminal,then the protein was combined to Sepharose 4B activated by CNBr in order to get the anti-H-Y antibody.Result:After purification,SMCY reeombined protein achieved to a high level more than 80% purity,and the specified IgY antibody achieves to 97%.By immunohistochemical assay,our purified anti-H-Y antibody could combine to the mouse liver cells of male... |
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| Keywords: | H-Y Ni-NTA IgY |
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