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用基因操作法改良糖甜菜(英)
引用本文:埃.,MC.用基因操作法改良糖甜菜(英)[J].中国糖料,1996(1):23-31.
作者姓名:埃.  MC
作者单位:( MC Elliott,DF Chen,MR Fowler,MJ Kirby,M Kubalakova,NW Scott,CL Zhang and A Slater The Norman Borlaug Institute for Plant Science Research,De Montfort University,Scraptoft,Leicester,LE7 9SU,UK
摘    要:甜菜的产糖量占世界蔗糖产量的40%,从碳同化角度来说,甜菜是极高效的温带作物之一,但用基因操作法仍有提高其产质量的潜力。对甜菜贮藏器官发育过程的解剖、生理和蔗糖积累的研究,为制定增加产量和减少环境对甜菜生长影响的策略奠定了理论基础。为促进对发育操作过程以达到上述这些目标,必须增强对细胞分裂的调节机制的认识。我们先研究植物激素限定细胞分裂的方式,随后研究调节细胞分裂的一些分子生物学特征。目前,我们已分离和鉴定了一些与甜菜细胞周期相关的基因序列,并用甜菜细胞悬浮培养对这些与细胞分裂调节相关的基因表达进行了研究,细胞分裂调节基因特定位点表达的策略已被确定,进一步优化甜菜转化系统的研究正日趋完善。通过这个途径,有希望获得高根产、高含糖、高汁液纯度和低环境影响的甜菜植株。

关 键 词:糖甜菜,基因操作法,细胞,细胞分裂

Towards the Perfect Sugar Beet via Gene Manipulation
Institution:( MC Elliott,DF Chen,MR Fowler,MJ Kirby,M Kubalakova,NW Scott,CL Zhang and A Slater The Norman Borlaug Institute for Plant Science Research,De Montfort University,Scraptoft,Leicester,LE7 9SU,UK)
Abstract:Sugar bcet provides almost 40%of the world' s sucrose.Although it ls one of the most efficicnt temperate zone crops in terms of carbon assimilation there is still the potential to enhance its qualities by gene manipulation. Studies of the anatomy and physiology of storage organ development and sucrose accumulation provide the basis for a strategy to increase sucrose yields and reduce the environmental impact of crop growth.To facilitate the manipulation of development so as to achieve these ends it has been necessary to in- crease understanding of the regulation of sugar beet cell division,An investigation of the phytohormonal determi- nation of division patterns was followed by studies of certain molecular biological aspeets of the rbgulatory cas-cade. Several cell cycle related gene sequences from sugar beet have been isolated and characterised. The regula-tion of cell division related gene expression has been studied in sugar beet cell suspension cultures.A strategy for site-specific expression of cell division regulating genes has been defined and a transformation system for sugar beet is being optimised.The route towards high root yield/ high sucrose coneentration/ high juice purity/low en- vironmental impact sugar beet plants is now clear.
Keywords:Sugar beet Gene manipulation Cell Cell division
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