| 基于NCBI 数据库的油棕EST-SSR
标记的开发与应用 |
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| 引用本文： | 夏薇,肖勇,杨耀东,沈雁,雷新涛,马子龙.基于NCBI 数据库的油棕EST-SSR
标记的开发与应用[J].广东农业科学,2014,41(2):144-148. |
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| 作者姓名： | 夏薇 肖勇 杨耀东 沈雁 雷新涛 马子龙 |
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| 作者单位： | 1.中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室袁海南文昌571339
2.中国热带农业科学院热带生物技术研究海南海口571101 |
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| 基金项目： | 国家自然科学基金31101179 |
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| 摘 要： | 应用NCBI 网站上释放的41 977 条油棕EST 序列，从中挖掘SSR 位点3 946 个，根据27 个SSR 位点的
侧翼序列设计引物，并对18 份油棕DNA 样品进行扩增，扩增结果显示10 个标记具有多态性。根据这些标记信息，
对这18 份油棕资源进行主成分分析，18 份油棕资源被分成两个遗传群体，一个为文昌高隆湾和文昌椰子研究所的
油棕资源，另一个群体为10 份引自马来西亚的油棕资源。这些研究显示，开发的标记可用于油棕资源遗传多样性评
估、遗传结构评估，并可用于油棕遗传图谱的构建。
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| 关 键 词： | 油棕 SSR 多态性 平均遗传距离 遗传结构 |
Development and utilization of SSR markers
of oil palm according to NCBI database |
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| Abstract: | Applying 41977 expressed sequence tags (EST) of oil palm released in NCBI, 3946 SSR loci were
identified. We designed primers for 27 SSR loci based on their flanking sequences and conducted amplification in 18 oil
palm DNA samples. The amplification result showed that 10 SSR markers were polymorphic. According to the ten SSR
makers, we used Principle Component Analysis to elucidate population structure of the 18 oil palm germplasm. Principle
Component Analysis demonstrated that the 18 oil palm germplasm could be classified into two genetic groups: one was
composed of oil palm germlasm collected from Gaolong bay and coconut institution in Wenchang city; the other including
germplasm introduced from Malaysia. This study showed that developed SSR markers could be used for characterization of
genetic diversity, population structure and construction of genetic map for oil palm. |
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| Keywords: | oil palm SSR polymorphism average genetic distance genetic structure |
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