| ~(60)Co-γ辐射诱变对康氏木霉产纤维素酶的影响 |
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| 引用本文： | 林元山,陈桂光,梁智群,王定军.~(60)Co-γ辐射诱变对康氏木霉产纤维素酶的影响[J].安徽农业科学,2007,35(24):7567-7568. |
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| 作者姓名： | 林元山 陈桂光 梁智群 王定军 |
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| 作者单位： | 1. 广西大学生命科学院,广西南宁,530004;湖南农业大学生命科学院,湖南长沙,410128
2. 广西大学生命科学院,广西南宁,530004 |
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| 基金项目： | 国家自然科学基金项目(20066001) |
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| 摘 要： | 为了研究不同剂量60Co-γ辐射对康氏木霉产纤维素酶的影响。以康氏木霉AS3.2774为出发菌株,通过0.6~1.8 Gy辐照剂量的60 Co-γ辐射诱变康氏木霉,经平板培养和固体发酵培养筛选出高产菌株,研究不同剂量60Co-γ辐射处理对康氏木霉孢子存活率、康氏木霉产纤维素酶发酵能力和遗传稳定性的影响。结果表明,辐照剂量在0.9~1.1 Gy,康氏木霉孢子致死率达到70.0%~80.0%,辐照剂量大于1.5 Gy时,存活率接近0。辐照处理的孢子经刚果红平板初筛、固体发酵复筛、遗传稳定性试验,获得4株正突变菌株。突变株产纤维素酶活力平均数与出发菌株间存在显著差异,其中菌株Ly-23产纤维素酶的能力平均达0.356 IU/ml,比出发菌株提高了31.8%。该研究为从康氏木霉中筛选纤维素酶高产菌株奠定了基础。
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| 关 键 词： | 60Co-γ辐照 纤维素酶 康氏木霉 |
| 文章编号： | 0517-6611（2007）24-07567-02 |
| 修稿时间： | 2007-04-18 |
Effect of 60 Co γ-irradiation on Production of Cellulase from Trichoderma knoningii |
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| LIN Yuan-shan et al.Effect of 60 Co γ-irradiation on Production of Cellulase from Trichoderma knoningii[J].Journal of Anhui Agricultural Sciences,2007,35(24):7567-7568. |
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| Authors: | LIN Yuan-shan |
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| Abstract: | The effect of 60 Co γ-irradiation,with dose 0.6～1.8 Gy,on production of cellulase from Trichoderma knoningii AS3.2774 was studied.The results indicated the death rate of spores reached 70.0%～80.0% when radiation dosage was 0.9～1.1 Gy.After irradiation,the strains were screened firstly by congo red plate;then solid fermentation,and finally experiment in th estability of genetic transfer of cellulase production.It was showed 4 positive mutants had significant difference comparative to the original with paired samples test(t-test).The filter paper activity of cellulase of strain Ly-23 reached 0.356 IU/ml,with 1.8% enhanced,compared with initial strain. |
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| Keywords: | 60 Co γ-irradiation Cellulase Trichoderma knoningii |
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