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| AsA-GSH循环对水稻突变体耐汞性的作用 | |
| 引用本文： | 曾斌,王飞娟,朱诚,孙宗修.AsA-GSH循环对水稻突变体耐汞性的作用[J].作物学报,2008,34(5):823-830. |
| 作者姓名： | 曾斌 王飞娟 朱诚 孙宗修 |
| 作者单位： | 1 浙江大学生命科学学院植物生理学与生物化学国家重点实验室, 浙江杭州310058; 2 中国水稻研究所水稻生物学国家重点实验室, 浙江杭州310006 |
| 基金项目： | 国家科技支撵计划项目 , 浙江省自然科学基金 , 国家重点基础研究发展计划(973计划) |
| 摘 要： | 以水稻中花11及其汞耐性突变体的溶液培养, 研究了在0.4 mmol L-1 Hg2+胁迫下汞耐性突变体的活性氧代谢及抗坏血酸-谷胱甘肽(AsA-GSH)循环的变化。结果表明, Hg2+胁迫下, 突变体叶片中的H2O2含量、产生速率和MDA含量、GSSG含量、DHA含量显著低于野生型; 突变体叶片中GSH/GSSG和AsA/DHA的比值均高于野生型, 同时突变体根和茎中的Hg2+含量明显高于野生型。说明汞耐性突变体体内AsA-GSH循环在Hg2+胁迫下仍能保持较高的运转效率和清除活性氧的能力, 在其耐汞性中起重要作用。 |
| 关 键 词： | Hg2+ 水稻 突变体 活性氧 抗坏血酸-谷胱甘肽循环 |
| 文章编号： | 10.3274/SP.J.1006.2008.00823 |
| 收稿时间： | 2007-09-23 |
| 修稿时间： | 2007年9月23日 |
Effect of AsA-GSH Cycle on Hg2+-Tolerance in Rice Mutant |
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| ZENG Bin,WANG Fei-Juan,ZHU Cheng,SUN Zong-Xiu.Effect of AsA-GSH Cycle on Hg2+-Tolerance in Rice Mutant[J].Acta Agronomica Sinica,2008,34(5):823-830. | |
| Authors: | ZENG Bin WANG Fei-Juan ZHU Cheng SUN Zong-Xiu |
| Institution: | 1.State Key Laboratory of Plant Physiology & Biochemistry, College of Life Science, Zhejiang University, Hangzhou 310058, Zhejiang;2.State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, Zhejiang, China |
| Abstract: | Mercury (Hg) toxicity is an in rice growth problem throughout the world. In the present study, Zhonghua 11 and a Hg2+-tolerant rice mutant (MT) were used in a solution culture to investigate the effect of 0.4 mmol L-1 Hg2+ treatment on reactive oxygen species (ROS) metabolism and dynamic change of ascorbate-glutathione (AsA-GSH) cycle. The results indicated that the H2O2 content, evolution rate, MDA content, GSSG and DHAcontents of the leaves were higher in the wild type than in the mutant; both the ratio of GSH to GSSG and the ratio of AsA to DHA were higher in the mutant than in the wild type, while the accumulation of Hg2+ in roots and stems of the mutant was more than that of the wild type. The results reveal that AsA-GSH cycle was less inhibited in the mutant than in the wild type, thus the mutant was able to scavenge ROS more. An effective AsA-GSH cycle is important for the mercury resistance of mutant. |
| Keywords: | Hg2+ Rice Mutant Reactive oxygen species AsA-GSH cycle |
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