| 降低苍溪梨外植体组培褐变途径的研究 |
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| 引用本文： | 李焕秀,乔霓娇.降低苍溪梨外植体组培褐变途径的研究[J].西南农业大学学报,2001,23(6):524-526. |
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| 作者姓名： | 李焕秀 乔霓娇 |
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| 作者单位： | 四川农业大学林学园艺学院,四川雅安625014 |
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| 基金项目： | 四川省科委应用基础资助项目(7210) |
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| 摘 要： | 以苍溪梨为试材进行降低梨外植体组培褐变研究。结果表明,苍溪梨适宜在MS＋1．0mg/LBA 0.02mg/LNAA上生长分化,将外植体在0．002mol/L的8－羟基奎啉溶液中作不同时间的处理,20min处理更有利于防止褐变同时不阻抑梨芽生长,成活率高达90％,2h处理时成活率降低为0。从3月到10月用相同的培养基进行培养,4月下旬采集的材料的褐变率最高,5月份以后外植体组培褐变率逐渐降低,10月份降低为0。通过不同的褐变抑制剂预处理（从田间产样到接种前的褐变抑制剂浸泡）试验表明,在田间将采集的梨外植体进行低温处理,接种前作20min8－羟基奎啉和苯甲酸钠（浓度分别为0．002mol/L和0．2mol/L)浸泡处理对降低褐变和提高成活率均有较为理想的效果。
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| 关 键 词： | 苍溪梨 外殖体 组培褐变 梨 |
| 文章编号： | 1000-2642(2001)06-0524-03 |
| 修稿时间： | 2001年3月13日 |
APPROACHES FOR REDUCING ESPLANT BROWNING OF "CANGXILI"PEAR(PYRUS RYRIFOLIA NAKAI)IN TISSURE CULTURE |
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| LI Huan-xiu,QIAO Ni-jiao.APPROACHES FOR REDUCING ESPLANT BROWNING OF "CANGXILI"PEAR(PYRUS RYRIFOLIA NAKAI)IN TISSURE CULTURE[J].Journal of Southwest Agricultural University,2001,23(6):524-526. |
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| Authors: | LI Huan-xiu QIAO Ni-jiao |
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| Abstract: | The explants of "Cangxili", a pear ( Pyrus pyrifolia Nakai) genotype, were found to grow well on the medium MS 1.0 mg/L BA 0.02 mg/L NAA. The explants were soaked in a solution containing 8-hydroxyquinoline (8-HQS) for different time duration, the treatment for 20 min gave the most satisfactory results: it prevented the browning of the explants and had no inhibiting effect of their growth, with a browning rate of 10% and a survival rate of 90%. In contrast, 8-HQS treatment for 2 hr resulted in a 100% browning and 0% survival. Survival rate varied with the season of explant collection. Highest browning rate occurred in explants collected in April. From May on, the browning rate decreased, and it was reduced to 0 in October. In antioxidant treatments, lower temperature plus 20 min 8-HQS (0.002 mg/L) or sodium benzoate (0.2 mg/L) before inoculation appeared to be optimum for reducing browning rate and improving survival of the explants. |
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| Keywords: | Cangxili (Pyrus pyrifolia Nakai) expalnt browning in vitro |
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