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Bacterial community structure in lake sediments of microcosms contaminated with nonylphenol
Authors:Miriam Jontofsohn  Gerd Pfister  Gabriele Severin  Karl-Werner Schramm  Anton Hartmann  Michael Schloter
Institution:1. GSF — National Research Center for Environment and Health, Institute of Soil Ecology, Ingolst?dter Landstr.l, D-85764, Neuherberg, Germany
2. GSF — National Research Center for Environment and Health, Institute of Ecological Chemistry, Ingolst?dter Landstr.l, D-85764, Neuherberg, Germany
Abstract:The impact of nonylphenol, an estrogenic degradation product of alkylphenol polyethoxylates, on the bacterial community structure in contaminated sediments of aquatic microcosms was investigated over a period of 20 weeks using a 16S rDNA-based molecular phylogenetic approach. All microcosms showeda strong seasonal fluctuation of the dominant as well as the active bacterial microflora independent of their degree of contamination with nonylphenol. These changes were correlated with the dynamic of the total organic carbon content (TOC), ranging from 4–39 g/kg sediment dry weight and the redox potential in the sediment. Even at the highest observed nonylphenol concentration (3.4 mg/kg sediment dry weight) the bacterial community structure was mostly unchanged.
Keywords:16S rRNA-fingerprint  ecotoxicology  microbial community structure  nonylphenol
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