| 棉纤维发育重要基因FbL2A的SNPs研究及定位 |
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| 引用本文： | 韩志国,楚鹰,郭旺珍,张天真.棉纤维发育重要基因FbL2A的SNPs研究及定位[J].农业生物技术学报,2006,14(3):360-364. |
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| 作者姓名： | 韩志国 楚鹰 郭旺珍 张天真 |
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| 作者单位： | 南京农业大学作物遗传与种质创新国家重点实验室,棉花研究所,南京,210095 |
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| 基金项目： | 科技部科研项目;高比容电子铝箔的研究开发与应用项目;转基因植物专项基金;教育部长江学者奖励计划;教育部科学技术研究项目 |
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| 摘 要： | 利用PCR技术在7个四倍体棉种(Gossypium hirsutum L.和G.barbadense L.)和2个二倍体祖先棉种(G. herbaceum L.和G. richmondii U.)中扩增出棉纤维发育重要基因FbL2A, 分析了不同材料间存在的单核苷酸多态性(single nucleotide polymorphisms, SNPs)。根据TM-1和海7124两个材料中FbL2A基因的测序结果, 采用NEBcutter软件分析, 选择BstUⅠ内切酶酶解扩增产物, 利用Mapmaker v3.0b作图软件将FbL2A基因定位到第14染色体。另对该基因在4个陆地棉高强纤维品系间的SNPs位点进行了分析，发现编码区的SNPs位点中存在非同义突变，可能与陆地棉纤维品质等表型相关。
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| 关 键 词： | 棉纤维发育基因FbL2A 单核苷酸多态性 酶切扩增多态性 基因定位 |
| 文章编号： | 1006-1304（2006）03-0360-03 |
| 收稿时间： | 2005-5-13 |
| 修稿时间： | 2006-5-19 |
Divergence and Mapping of SNPs of the FbL2A Gene for Cotton Fiber Development |
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| HAN Zhi-guo,CHU Ying,GUO Wang-zhen,ZHANG Tian-zhen.Divergence and Mapping of SNPs of the FbL2A Gene for Cotton Fiber Development[J].Journal of Agricultural Biotechnology,2006,14(3):360-364. |
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| Authors: | HAN Zhi-guo CHU Ying GUO Wang-zhen ZHANG Tian-zhen |
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| Institution: | National Key Laboratory of Crop Genetics and Germplasm Enhancement Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Using PCR-based method, FbL2A gene was isolated from seven allotetraploid cotton (Gossypium hirsutum L. and G. barbadense L.) materials with different fiber quality and two diploid progenitors (G.herbaceum L. and G. richmondii U.). Based on single nucleotide polymorphisms (SNPs) of the FbL2A gene sequences in TM-1 and H-7124, the amplified products were digested by BstUⅠ through NEBcutter software online. And the gene was mapped on the chromosome 14 by Mapmaker v3.0b mapping software. In the accessions of upland cotton (G. hirsutum) with high fiber quality, the coding region SNPs with non-synonymous substitute were found, which may have phenotype effect. |
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| Keywords: | FbL2A SNPs CAPs gene mapping |
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