| DNA微阵列与基因表达研究概况 |
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| 引用本文： | 刘丽玲,王秀荣,陈化兰.DNA微阵列与基因表达研究概况[J].动物医学进展,2003,24(6):15-18. |
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| 作者姓名： | 刘丽玲 王秀荣 陈化兰 |
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| 作者单位： | 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室兽医生物技术国家重点实验室,黑龙江哈尔滨,150001 |
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| 基金项目： | 国家自然科学基金项目 (30 2 0 0 2 0 1) |
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| 摘 要： | DNA微阵列技术是 90年代兴起的一种对成百上千甚至上万个基因同时进行检测的新技术 ,它具有高通量和并行化的特点 ,广泛应用于基因表达、预测基因功能、检测基因突变和多态性分析、发现新药物和药物靶器官以及疫苗设计等方面。文章对 DNA微阵列的基本原理、DNA微阵列制备技术、杂交信号检测以及数据分析。 c DNA微阵列与细胞周期相关基因表达、细菌基因表达、病毒基因表达、肿瘤基因表达进行了概述。
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| 关 键 词： | DNA 微阵列 基因表达 |
| 文章编号： | 1007-5038(2003)06-0015-04 |
| 修稿时间： | 2003年4月30日 |
DNA Microarray and Gene Expression |
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| LIU Li-ling,WANG Xiu-rong,CHEN Hua-lan.DNA Microarray and Gene Expression[J].Progress In Veterinary Medicine,2003,24(6):15-18. |
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| Authors: | LIU Li-ling WANG Xiu-rong CHEN Hua-lan |
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| Abstract: | DNA microarray is a new technology which can detect tens of thousands of genes simultaneously in a high-throughout and parallel manner. It was widely used in gene expression, gene function prophet, gene mutation and polymorphism screening,discovery of drugs and their targets and vaccine design. The basic principle of DNA microarray is nucleic acid hybridization. Advancements in the technology include microarrays fabrication,signal detection,data analysis. The study on gene expression in various states,is one of the most important applicants of DNA microarray. cDNA microarray is related with cell cycle-related gene expression,bacteria gene expression,virus gene expression and tumor gene expression. This review detailed the principle and applications of cDNA microarray in the field of gene expression. |
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| Keywords: | DNA microarray gene expression |
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