Seasonal cycle and regulation by temperature of antifreeze protein mRNA in a Long Island population of winter flounder |
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| Authors: | Jeffrey L Price Catherine E Lyons Ru Chih C Huang |
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| Institution: | (1) Department of Biology, The Johns Hopkins University, Baltimore, Maryland, 21218;(2) Institute of Molecular Biology, Academia Sinica, Nankang, Taipei, 11529, Republic of China |
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| Abstract: | The seasonal cycle and regulation by temperature of antifreeze protein mRNA (AF mRNA) were investigated in a Long Island population
of winter flounder (Pseudopleuronectes americanus) by Northern blot hybridization and by in vitro translation of liver RNA. AF mRNA was expressed at high levels in the fall and winter (Nov.–Feb.) and at low or undetectable
levels in the summer. The time of accumulation of AF mRNA coincides with the time during which water temperature and photoperiod
decrease to 4°C and 9 h of light per day, respectively. A temperature and photoperiod decrease in the laboratory during this
time also resulted in high levels of AF mRNA. The levels of other mRNAs, as assayed by in vitro translation, were relatively constant during both seasonal acclimation and laboratory acclimation. The seasonal cycle of
AF mRNA in Long Island winter flounder is similar to that of a more northern, Newfoundland population of winter flounder and
different from that of an intermediate, New Brunswick population. These similarities and dissimilarities are discussed in
light of potentially different exogenous and endogenous regulatory cues in the different populations. |
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| Keywords: | serum liver Pseudopleuronectes americanus regulation by temperature Northern blot in vitro translation |
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