| 河北省甘薯镰孢菌腐烂与溃疡病的病原鉴定 |
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| 引用本文： | 王容燕,高波,陈书龙,马娟,李秀花.河北省甘薯镰孢菌腐烂与溃疡病的病原鉴定[J].植物保护学报,2016,43(2):241-247. |
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| 作者姓名： | 王容燕 高波 陈书龙 马娟 李秀花 |
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| 作者单位： | 河北省农林科学院植物保护研究所,河北省农业有害生物综合防治工程技术研究中心,农业部华北北部作物有害生物综合治理重点实验室,保定071000 |
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| 基金项目： | 国家现代农业(甘薯)产业技术体系(CARS-11-B-08),河北省财政基本科研业务费(494-0401-JBN-6440) |
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| 摘 要： | 为明确河北省甘薯镰孢菌腐烂与溃疡病的症状特点和病原种类,在不同种植区采集储藏期和育秧期病样,描述其症状特征;对病原菌进行分离纯化,采用柯赫氏法则回接验证,依据病原菌的形态特征和基因序列确定病原菌种类。结果表明,在储藏期甘薯发病可导致薯块表面腐烂的占总病薯的59.09%,端部腐烂的占40.91%;薯块带有褐色轮纹病斑的占61.36%,病斑没有轮纹或者轮纹不明显的占38.64%;发病初期薯块内部病斑浅、黑褐色的占27.27%,发病后期薯块内部形成空腔、布满白色菌丝的占72.73%;病斑带有苦味的占59.09%,病斑没有苦味或苦味不明显的占40.91%;在育秧田发病导致薯秧溃疡,表现为主茎基部呈点片发生黑色或者褐色病斑,部分有开裂。分离的病原菌能够同时侵染薯块和薯秧;病原菌单瓶梗产孢,大型分生孢子稍弯,两端钝圆,多数3~7个分隔,顶细胞钝圆,基细胞足跟较明显。其rDNA-ITS、EF-1α、β-tubulin基因序列与茄镰孢菌Fusarium solani的同源性分别为97%、99%和98%。初步确定甘薯镰孢菌腐烂与溃疡病的病原菌为茄镰孢菌F.solani。
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| 关 键 词： | 甘薯 镰孢菌腐烂与溃疡病 茄镰孢菌 症状 |
| 收稿时间： | 2014/10/3 0:00:00 |
Identification of the pathogens causing Fusarium root rot and stem canker on sweet potato in Hebei Province |
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| Wang Rongyan,Gao Bo,Chen Shulong,Ma Juan and Li Xiuhua.Identification of the pathogens causing Fusarium root rot and stem canker on sweet potato in Hebei Province[J].Acta Phytophylacica Sinica,2016,43(2):241-247. |
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| Authors: | Wang Rongyan Gao Bo Chen Shulong Ma Juan and Li Xiuhua |
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| Institution: | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China,Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China and Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Integrated Pest Management Centre of Hebei Province; Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China |
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| Abstract: | |
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| Keywords: | sweet potato Fusarium root rot and stem canker Fusarium solani symptom |
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