| 小麦品种百农64的慢白粉病QTL分析 |
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| 引用本文： | 王竹林,王德森,何中虎,王辉,陈新民,段霞瑜,周益林,夏先春.小麦品种百农64的慢白粉病QTL分析[J].中国农业科学,2006,39(10):1956-1961. |
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| 作者姓名： | 王竹林 王德森 何中虎 王辉 陈新民 段霞瑜 周益林 夏先春 |
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| 作者单位： | 中国农业科学院作物科学研究所 |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | 白粉病是我国小麦的主要病害，发掘慢白粉抗病基因及其紧密连锁的分子标记是培育慢病性品种的基础。本实验利用100个SSR标记和58个AFLP标记，分析百农64×京双16组合218个F2:3系的基因型，构建了由158个位点组成的遗传连锁图，在小麦连锁群上覆盖3114 cM。2003-04和2004-05年度将该群体种植于北京和安阳两点，鉴定各个家系对白粉病的抗性。采用复合区间作图法进行慢白粉病的QTL分析，发现3个控制慢白粉病的QTL，其中位于2B和2D 染色体的2个QTL在两种环境下均能检测到，贡献率分别为9.6%～11.3%和21.2%~26.1%。QTL遗传效应表现为加性和部分显性，其加性效应均来自抗病亲本百农64。
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| 关 键 词： | 普通小麦 白粉病 慢病性 QTL分析 |
| 收稿时间： | 2005-11-11 |
| 修稿时间： | 2005-11-112006-03-20 |
QTL Mapping for Slow Mildewing Resistance in Chinese Wheat Cultivar Bainong 64 |
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| WANG Zhu-lin,WANG De-sen,HE Zhong-hu,WANG Hui,CHEN Xin-min,DUAN Xia-yu,ZHOU Yi-lin,XIA Xian-chun.QTL Mapping for Slow Mildewing Resistance in Chinese Wheat Cultivar Bainong 64[J].Scientia Agricultura Sinica,2006,39(10):1956-1961. |
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| Authors: | WANG Zhu-lin WANG De-sen HE Zhong-hu WANG Hui CHEN Xin-min DUAN Xia-yu ZHOU Yi-lin XIA Xian-chun |
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| Abstract: | Powdery mildew is one of the most serious wheat diseases in China. Identification of slow mildewing resistance genes and their closely linked molecular markers is very important for developing wheat cultivars with durable resistance. An F2:3 population with 218 lines derived from the cross between Bainong 64 and Jingshuang 16 were sown with three replicates in Beijing and Anyang, Henan Province. Inoculation was carried out with the isolate E20 of B. graminis f. sp. tritici before stem elongation, and disease severity to powdery mildew was scored for 4 times until the leaves were physiologically mature in Beijing during 2003-04 and 2004-05 cropping seasons. In Anyang, disease was evaluated around May 18, in 2004-05 cropping season under natural inoculation. A total of 100 SSR markers and 58 AFLP markers were used to construct a linkage map, covering 3114 cM of wheat linkage groups except chromosome 1D. With the method of composite interval mapping (CIM), three QTLs were found on chromosomes 2B, 2D and 4D, in which the QTLs on chromosomes 2B and 2D were detected across two environments, accounting for 9.6%~11.3% and 21.2%~26.1% of phenotypic variance, respectively. The QTLs showed additive and partially dominant effect and the additive effects were derived from the resistant parent Bainong 64. The molecular markers identified in this study will certainly benefit for marker-assisted selection and pyramiding of resistance genes to powdery mildew in wheat breeding programs. |
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| Keywords: | Bread wheat(Triticum aestivum L ) Powdery mildew Slow mildewing resistance QTL mapping |
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