| 甘蓝型油菜小孢子培养研究 |
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| 引用本文： | 李浩杰,蒲晓斌,张锦芳,蒋俊,蒋梁材.甘蓝型油菜小孢子培养研究[J].农业科学与技术,2009,10(4):37-40. |
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| 作者姓名： | 李浩杰 蒲晓斌 张锦芳 蒋俊 蒋梁材 |
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| 作者单位： | 四川省农业科学院作物研究所,四川成都,610066 |
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| 摘 要： | 1材料与方法
1．1材料与培养基以甘蓝型油菜NER为试材，分别于2006年9月、2007年9月、2008年9月种植于四川省农科院郫县农场。提取液（B5）：B5大量（KN032500mg／L＋MgSq250mg／L＋CaCl2·2H2O150mg／L＋NaH2PO4·H2O150mg／L）＋B5微量Ⅰ＋B5微量Ⅱ＋KI1000mg／L＋B5有机＋Fe—Salt肌醇100mg／L＋蔗糖130g／L（高压灭菌，置4℃冰箱中备用）。诱导基本培养基（NLN·13）：NLN-13大量＋MS微量Ⅰ＋NLN-13微量Ⅱ＋Fe—EDTA40．0mg／L＋甘氨酸2．0mg／L＋烟酸5．0mg／L＋VB60．5mg／L＋VB．0．5mg／L＋肌醇100．0mg／L＋生物素0．5mg／L＋叶酸0．5mg／L＋L-丝氨酸100．0mg／L＋谷氨酰胺800．0mg／L＋谷胱甘肽30．0mg／L＋蔗糖13％＋NAA0．5mg／L＋6-BA0．05mg／L。分化培养基MS：MS＋蔗糖2％＋琼脂粉6g／L。
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| 关 键 词： | 甘蓝型油菜 小孢子培养 基本培养基 L-丝氨酸 分化培养基 高压灭菌 谷胱甘肽 谷氨酰胺 |
Studies on Enhancing Yield of Embryos in Microspore Culture of Brassica napus L. |
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| LI Hao-jie,PU Xiao-bin,ZHANG Jin-fang,JIANG Jun,JIANG Liang-cai.Studies on Enhancing Yield of Embryos in Microspore Culture of Brassica napus L.[J].Agricultural Science ＆ Technology,2009,10(4):37-40. |
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| Authors: | LI Hao-jie PU Xiao-bin ZHANG Jin-fang JIANG Jun JIANG Liang-cai |
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| Institution: | ( Crop Institute of Sichuan Academy of Agricultural Sciences, Chengdu 610066) |
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| Abstract: | In ecological zone of Chengdu, Sichuan, microspore culture was carried out in Brassica napus L. to study the influencing factors on microspore culture. The results showed that the temperature on microspore formation stage, day and night temperature, disinfection solution of buds, cultivation concentration on microspore and strain-age were both important influencing factors on microspore culture. At a temperature below 5 ℃ or above 20 ℃, the material had a much lower embryo producing rate or even could not produce any embryo, but at the optimum temperature of 10 -15 ℃ the embryo yield was up to 300 pieces per bud; the best cultivation effect appeared when 0. 1% HgCl2 was used for disin- fection; the best density of microspore was 3 -4 buds per dish; In 2009, while strain-age was from 125 d to 150 d, the microspore embryo yield increased as strain-age increased. When stain-age was 150 days, the microspore embryo yield was up to the highest, but the yield declined after 150 days. |
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| Keywords: | Brassica napus L Isolated microspore culture Technical system Stain-age Embryo yield |
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