| 基因组时代荧光原位杂交技术在家蚕研究中的应用 |
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| 引用本文： | 沈以红.基因组时代荧光原位杂交技术在家蚕研究中的应用[J].蚕学通讯,2006,26(3):27-35. |
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| 作者姓名： | 沈以红 |
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| 作者单位： | 西南大学蚕学重点实验室,重庆,400716 |
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| 基金项目： | 重庆市自然科学基金项目. |
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| 摘 要： | 随着家蚕DNA分子标记和物理图谱的发展,基因组序列的释放,以及EST数据库和BAC文库的构建,FISH技术在家蚕的遗传学、基因组学研究,尤其是物理图谱研究中取得重要进展。本文综述了FISH及相关技术原理及近年在家蚕和部分鳞翅目昆虫研究中的应用,并对其在家蚕及鳞翅目昆虫的研究前景作了展望。
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| 关 键 词： | 荧光原位杂交 基因组 家蚕 |
APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION IN THE STUDY OF SILKWOR, BOMBYX MORI, IN THE GENOMIC ERA |
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| SHEN Yihong.APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION IN THE STUDY OF SILKWOR, BOMBYX MORI, IN THE GENOMIC ERA[J].Newsletter of Sericultural Science,2006,26(3):27-35. |
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| Authors: | SHEN Yihong |
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| Institution: | Key Sericultural Laboratory of Southwest University, Chongqing 400716, China |
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| Abstract: | With the development of DNA molecular markers and physical mapping, the release of whole genome sequence and the construction of EST databases and BAC libraries, significant advances have been made in recent years in the application of FISH (fluorescence in situ hybridization) in the research areas of genetics and genomics of silkworm, Bombyx mori, particularly in its physical mapping. This paper reviews the principles of FISH and related technologies and their application in the study of silkworm and some other insects of Lepidoptera and discusses their potential uses in the future. |
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| Keywords: | fluorescence in situ hybridization (FISH) genome Bombyx mori |
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